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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFAIP8L2 Recombinant antibody (85759-4-RR)

    WB analysis using 85759-4-RR (same clone as 85759-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85759-4-RR (TNFAIP8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85759-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using TNFAIP8L2 Recombinant antibody (85759-4-RR)

    IHC staining of mouse spleen using 85759-4-RR (same clone as 85759-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85759-4-RR (TNFAIP8L2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85759-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using TNFAIP8L2 Recombinant antibody (85759-4-RR)

    IHC staining of mouse spleen using 85759-4-RR (same clone as 85759-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85759-4-RR (TNFAIP8L2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85759-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85759-4-RR

    Affinity and Kinetic Characterization of 85759-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85759-4-RR against Human TNFAIP8L2 were performed. The affinity constant is below 1 pM.

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TNFAIP8L2 Recombinant antibody, PBS Only

TNFAIP8L2 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85759-4-PBS
Clone No.250047D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

TIPE2, Inflammation factor protein 20, TNF alpha-induced protein 8-like protein 2, TNFAIP8 like protein 2, TNFAIP8-like protein 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85759-4-PBS targets TNFAIP8L2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TNFAIP8L2 fusion protein Ag7964 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor, alpha-induced protein 8-like 2
Calculated molecular weight 184 aa, 21 kDa
Observed molecular weight18 kDa
GenBank accession numberBC063014
Gene Symbol TIPE2
Gene ID (NCBI) 79626
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P589
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TIPE2 (TNF-α-inducible protein 8-like 2, or TNFAIP8L2), a negative immune regulator, is essential for maintaining immune homeostasis. TIPE2 shares considerable sequence homology with members of the tumor necrosis factor-α-inducible protein 8 (TNFAIP8) family, which are thought to regulate cellular and immune homeostasis. TIPE2 acts as a negative regulator of Toll-like receptor and T-cell receptor function and is preferentially expressed in lymphoid tissues. TIPE2 plays an essential role in a signal transduction pathway that links the inflammatory immune response to specific conditions after cerebral ischemia