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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TIPE2/TNFAIP8L2 Recombinant monoclonal antibody (85759-4-RR)

    WB analysis using 85759-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85759-4-RR (TIPE2/TNFAIP8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using TIPE2/TNFAIP8L2 Recombinant monoclonal antibody (85759-4-RR)

    IHC staining of mouse spleen using 85759-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85759-4-RR (TIPE2/TNFAIP8L2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using TNFAIP8L2 Recombinant antibody (85759-4-RR)

    IHC staining of mouse spleen using 85759-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85759-4-RR (TNFAIP8L2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85759-4-RR

    Affinity and Kinetic Characterization of 85759-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85759-4-RR against Human TNFAIP8L2 were performed. The affinity constant is below 1 pM.

TIPE2/TNFAIP8L2 Recombinant monoclonal antibody

TIPE2/TNFAIP8L2 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85759-4-RR
Clone No.250047D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

TIPE2, Inflammation factor protein 20, TNF alpha-induced protein 8-like protein 2, TNFAIP8 like protein 2, TNFAIP8L2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells, RAW 264.7 cells, mouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85759-4-RR targets TIPE2/TNFAIP8L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7964

Product name: Recombinant human TNFAIP8L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-184 aa of BC063014

Sequence: MESFSSKSLALQAEKKLLSKMAGRSVAHLFIDETSSEVLDELYRVSKEYTHSRPQAQRVIKDLIKVAIKVAVLHRNGSFGPSELALATRFRQKLRQGAMTALSFGEVDFTFEAAVLAGLLTECRDVLLELVEHHLTPKSHGRIRHVFDHFSDPGLLTALYGPDFTQHLGKICDGLRKLLDEGKL

相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor, alpha-induced protein 8-like 2
Calculated molecular weight 184 aa, 21 kDa
Observed molecular weight18 kDa
GenBank accession numberBC063014
Gene Symbol TIPE2
Gene ID (NCBI) 79626
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ6P589
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TIPE2 (TNF-α-inducible protein 8-like 2, or TNFAIP8L2), a negative immune regulator, is essential for maintaining immune homeostasis. TIPE2 shares considerable sequence homology with members of the tumor necrosis factor-α-inducible protein 8 (TNFAIP8) family, which are thought to regulate cellular and immune homeostasis. TIPE2 acts as a negative regulator of Toll-like receptor and T-cell receptor function and is preferentially expressed in lymphoid tissues. TIPE2 plays an essential role in a signal transduction pathway that links the inflammatory immune response to specific conditions after cerebral ischemia

Protocols

Product Specific Protocols
IHC protocol for TIPE2/TNFAIP8L2 antibody 85759-4-RRDownload protocol
WB protocol for TIPE2/TNFAIP8L2 antibody 85759-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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