Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | Anti-CD3/CD28 treated human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-98056 targets TNF beta in FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0836 Product name: Recombinant Human TNF-beta protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 35-205 aa of BC034729 Sequence: LPGVGLTPSAAQTARQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQVVFSGKAYSPKATSSPLYLAHEVQLFSSQYPFHVPLLSSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPSTVFFGAFAL 相同性解析による交差性が予測される生物種 |
| Full Name | lymphotoxin alpha (TNF superfamily, member 1) |
| Calculated molecular weight | 205 aa, 22 kDa |
| GenBank accession number | BC034729 |
| Gene Symbol | TNF beta |
| Gene ID (NCBI) | 4049 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01374 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 TNF beta antibody CL594-98056 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |