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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using TMLHE Polyclonal antibody (16621-1-AP)

    WB analysis of human liver using 16621-1-AP (same clone as 16621-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 16621-1-AP (TMLHE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMLHE Polyclonal antibody (16621-1-AP)

    WB analysis using 16621-1-AP (same clone as 16621-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 16621-1-AP (TMLHE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TMLHE Polyclonal antibody (16621-1-AP)

    WB analysis of Jurkat using 16621-1-AP (same clone as 16621-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16621-1-AP (TMLHE antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using TMLHE Polyclonal antibody (16621-1-AP)

    IHC staining of human stomach using 16621-1-AP (same clone as 16621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16621-1-AP (TMLHE antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16621-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TMLHE Polyclonal antibody, PBS Only

TMLHE Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16621-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

TMLD, TML-alpha-ketoglutarate dioxygenase, Epsilon-trimethyllysine hydroxylase, Epsilon-trimethyllysine 2-oxoglutarate dioxygenase, EC:1.14.11.8

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16621-1-PBS targets TMLHE in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9919

Product name: Recombinant human TMLHE protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-376 aa of BC025269

Sequence: MWYHRLSHLHSRLQDLLKGGVIYPALPQPNFKSLLPLAVHWHHTASKSLTCAWQQHEDHFELKYANTVMRFDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIKPKTIRLDETTLFFTWPDGHVTKYDLNWLVKNSYEGQKQKVIQPRILWNAEIYQQAQVPSVDCQSFLETNEGLKKFLQNFLLYGIAFVENVPPTQEHTEKLAERISLIRETIYGRMWYFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGIQVFHCLKHEGTGGRTLLVDGFYAAEQVLQKAPEEFELLSKVPLKHEYIEDVGECHNHMIGIGPVLNIYPWNKELYLIRLFKEKQNTVNRQWNSSLQCDIPERILTYRHFVSGTSIEHRGSLI

相同性解析による交差性が予測される生物種
Full Name trimethyllysine hydroxylase, epsilon
Calculated molecular weight 376 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC025269
Gene Symbol TMLHE
Gene ID (NCBI) 55217
RRIDAB_2205303
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVH6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Trimethyllysine dioxygenase (TMLHE) encodes the first enzyme in carnitine biosynthesis, N6-trimethyllysine dioxygenase (TMLD) and enzyme deficiency was suggested to be a risk factor for ASD.

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