Validation Data Gallery
Tested Applications
| Positive WB detected in | human liver tissue, rat kidney tissue, Jurkat cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
Product Information
16621-1-AP targets TMLHE in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9919 Product name: Recombinant human TMLHE protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-376 aa of BC025269 Sequence: MWYHRLSHLHSRLQDLLKGGVIYPALPQPNFKSLLPLAVHWHHTASKSLTCAWQQHEDHFELKYANTVMRFDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIKPKTIRLDETTLFFTWPDGHVTKYDLNWLVKNSYEGQKQKVIQPRILWNAEIYQQAQVPSVDCQSFLETNEGLKKFLQNFLLYGIAFVENVPPTQEHTEKLAERISLIRETIYGRMWYFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGIQVFHCLKHEGTGGRTLLVDGFYAAEQVLQKAPEEFELLSKVPLKHEYIEDVGECHNHMIGIGPVLNIYPWNKELYLIRLFKEKQNTVNRQWNSSLQCDIPERILTYRHFVSGTSIEHRGSLI 相同性解析による交差性が予測される生物種 |
| Full Name | trimethyllysine hydroxylase, epsilon |
| Calculated molecular weight | 376 aa, 44 kDa |
| Observed molecular weight | 44 kDa |
| GenBank accession number | BC025269 |
| Gene Symbol | TMLHE |
| Gene ID (NCBI) | 55217 |
| RRID | AB_2205303 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NVH6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Trimethyllysine dioxygenase (TMLHE) encodes the first enzyme in carnitine biosynthesis, N6-trimethyllysine dioxygenase (TMLD) and enzyme deficiency was suggested to be a risk factor for ASD.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMLHE antibody 16621-1-AP | Download protocol |
| WB protocol for TMLHE antibody 16621-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Discov Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer.
| ||
Cell Rep Inborn Errors of Long-Chain Fatty Acid β-Oxidation Link Neural Stem Cell Self-Renewal to Autism.
| ||
Cell Chem Biol Controlled lipid β-oxidation and carnitine biosynthesis by a vitamin D metabolite. | ||
bioRxiv αKG-mediated carnitine synthesis promotes homologous recombination via histone acetylation | ||
Nat Commun BBOX1 restrains TBK1-mTORC1 oncogenic signaling in clear cell renal cell carcinoma |
