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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TMEM59 Polyclonal antibody (24134-1-AP)

    WB analysis of MCF-7 using 24134-1-AP (same clone as 24134-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24134-1-AP (TMEM59 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24134-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM59 Polyclonal antibody (24134-1-AP)

    IHC staining of human breast cancer using 24134-1-AP (same clone as 24134-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24134-1-AP (TMEM59 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24134-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM59 Polyclonal antibody (24134-1-AP)

    IHC staining of human breast cancer using 24134-1-AP (same clone as 24134-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24134-1-AP (TMEM59 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24134-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM59 Polyclonal antibody (24134-1-AP)

    IHC staining of human liver using 24134-1-AP (same clone as 24134-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 24134-1-AP (TMEM59 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24134-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM59 Polyclonal antibody (24134-1-AP)

    IHC staining of human liver using 24134-1-AP (same clone as 24134-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 24134-1-AP (TMEM59 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24134-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMEM59 Polyclonal antibody (24134-1-AP)

    IF Staining of HepG2 using 24134-1-AP (same clone as 24134-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 24134-1-AP (TMEM59 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 24134-1-PBS in a different storage buffer formulation.

TMEM59 Polyclonal antibody, PBS Only

TMEM59 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24134-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24134-1-PBS targets TMEM59 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21150

Product name: Recombinant human TMEM59 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-324 aa of BC016374

Sequence: MAAPKGSLWVRTQLGLPPLLLLTMALAGGSGTASAEAFDSVLGDTASCHRACQLTYPLHTYPKEEELYACQRGCRLFSICQFVDDGIDLNRTKLECESACTEAYSQSDEQYACHLGCQNQLPFAELRQEQLMSLMPKMHLLFPLTLVRSFWSDMMDSAQSFITSSWTFYLQADDGKIVIFQSKPEIQYAPHLEQEPTNLRESSLSKMSSDLQMRNSQAHRNFLEDGESDGFLRCLSLNSGWILTTTLVLSVMVLLWICCATVATAVEQYVPSEKLSIYGDLEFMNEQKLNRYPASSLVVVRSKTEDHEEAGPLPTKVNLAHSEI

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 59
Calculated molecular weight 36 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC016374
Gene Symbol TMEM59
Gene ID (NCBI) 9528
RRIDAB_2879439
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXS4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TMEM59 is a regulator of autophagy in response to bacterial infection. Overexpression of this protein has been found to promote apoptosis in a glioma cell line. Alternative splicing of TMEM59 results in multiple transcript variants.

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