TMEM59 Polyclonal antibody
TMEM59 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 24134-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
Product Information
24134-1-AP targets TMEM59 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TMEM59 fusion protein Ag21150 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 59 |
| Calculated molecular weight | 36 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC016374 |
| Gene symbol | TMEM59 |
| Gene ID (NCBI) | 9528 |
| RRID | AB_2879439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TMEM59 is a regulator of autophagy in response to bacterial infection. Overexpression of this protein has been found to promote apoptosis in a glioma cell line. Alternative splicing of TMEM59 results in multiple transcript variants.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM59 antibody 24134-1-AP | Download protocol |
| IHC protocol for TMEM59 antibody 24134-1-AP | Download protocol |
| IF protocol for TMEM59 antibody 24134-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress. | ||
J Extracell Vesicles A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells. |
