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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TMEM165 Polyclonal antibody (20485-1-AP)

    WB analysis of Jurkat using 20485-1-AP (same clone as 20485-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TMEM165 Polyclonal antibody (20485-1-AP)

    WB analysis of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using TMEM165 Polyclonal antibody (20485-1-AP)

    IP experiment of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

    IP result of anti-TMEM165 (IP:20485-1-AP, 4ug; Detection:20485-1-AP 1:1000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using TMEM165 Polyclonal antibody (20485-1-AP)

    IF Staining of Jurkat using 20485-1-AP (same clone as 20485-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TMEM165 antibody (20485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMEM165 Polyclonal antibody (20485-1-AP)

    IF Staining of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM165 antibody (20485-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMEM165 Polyclonal antibody (20485-1-AP)

    IF Staining of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM165 antibody (20485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

TMEM165 Polyclonal antibody, PBS Only

TMEM165 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 20485-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Putative divalent cation/proton antiporter TMEM165, TMPT27, TPARL, Transmembrane protein 165, Transmembrane protein PT27

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20485-1-PBS targets TMEM165 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14362

Product name: Recombinant human TMEM165 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 61-177 aa of BC003545

Sequence: VSTVLFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLLNGPGDVETGTSITVPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHC

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 165
Calculated molecular weight 324 aa, 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC003545
Gene Symbol TMEM165
Gene ID (NCBI) 55858
RRIDAB_10695184
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HC07
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TMEM165 is a putative divalent cation:proton antiporter that exchanges calcium or manganese ions for protons across the Golgi membrane. It mediates the reversible transport of calcium or manganese to the Golgi lumen driven by the proton gradient and possibly the membrane potential generated by V-ATPase, and provides calcium or manganese cofactors to resident Golgi enzymes and contributes to the maintenance of an acidic luminal Golgi pH required for proper functioning of the secretory pathway (PMID:22683087, 23569283, 27008884, 32047108). TMEM165 has two isoforms, with molecular weights of 28 kDa and 35 kDa respectively. It is also subject to ubiquitination modification, which may cause an increase in its molecular weight.

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