Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
20485-1-AP targets TMEM165 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14362 Product name: Recombinant human TMEM165 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 61-177 aa of BC003545 Sequence: VSTVLFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLLNGPGDVETGTSITVPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHC 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 165 |
| Calculated molecular weight | 324 aa, 35 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC003545 |
| Gene Symbol | TMEM165 |
| Gene ID (NCBI) | 55858 |
| RRID | AB_10695184 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HC07 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TMEM165 is a putative divalent cation:proton antiporter that exchanges calcium or manganese ions for protons across the Golgi membrane. It mediates the reversible transport of calcium or manganese to the Golgi lumen driven by the proton gradient and possibly the membrane potential generated by V-ATPase, and provides calcium or manganese cofactors to resident Golgi enzymes and contributes to the maintenance of an acidic luminal Golgi pH required for proper functioning of the secretory pathway (PMID:22683087, 23569283, 27008884, 32047108). TMEM165 has two isoforms, with molecular weights of 28 kDa and 35 kDa respectively. It is also subject to ubiquitination modification, which may cause an increase in its molecular weight.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TMEM165 antibody 20485-1-AP | Download protocol |
| IP protocol for TMEM165 antibody 20485-1-AP | Download protocol |
| WB protocol for TMEM165 antibody 20485-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis | ||
J Biol Chem The human Golgi protein TMEM165 transports calcium and manganese in yeast and bacterial cells. | ||
Oncol Rep TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity. | ||
Biochem Biophys Res Commun The Ca(2+)/H(+) antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca2+ATPase (SPCA1). | ||
J Hepatocell Carcinoma Identification of a Novel Prognostic Signature Based on N-Linked Glycosylation and Its Correlation with Immunotherapy Response in Hepatocellular Carcinoma |
