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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM111 Polyclonal antibody (24645-1-AP)

    WB analysis using 24645-1-AP (same clone as 24645-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24645-1-AP (TMEM111 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24645-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TMEM111 Polyclonal antibody (24645-1-AP)

    WB analysis of HEK-293 using 24645-1-AP (same clone as 24645-1-PBS)

    WB result of TMEM111 antibody (24645-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM111 transfected HEK-293 cells. This data was developed using the same antibody clone with 24645-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TMEM111 Polyclonal antibody (24645-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 24645-1-AP (same clone as 24645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24645-1-AP (TMEM111 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24645-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TMEM111 Polyclonal antibody (24645-1-AP)

    IHC staining of human ovary cancer using 24645-1-AP (same clone as 24645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24645-1-AP (TMEM111 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24645-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TMEM111 Polyclonal antibody (24645-1-AP)

    IF Staining of A431 using 24645-1-AP (same clone as 24645-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TMEM111 antibody (24645-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24645-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TMEM111 Polyclonal antibody, PBS Only

TMEM111 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24645-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EMC3, POB

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24645-1-PBS targets TMEM111 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18236

Product name: Recombinant human TMEM111 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 139-261 aa of BC022807

Sequence: KVPFPLTLRFKPMLQQGIELLTLDASWVSSASWYFLNVFGLRSIYSLILGQDNAADQSRMMQEQMTGAAMAMPADTNKAFKTEWEALELTDHQWALDDVEEELMAKDLHFEGMFKKELQTSIF

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 111
Calculated molecular weight 261 aa, 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC022807
Gene Symbol TMEM111
Gene ID (NCBI) 55831
RRIDAB_3085744
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0I2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TMEM111,also names as EMC3, is part of the endoplasmic reticulum-associated secretory pathway (PMID: 19797678). TMEM111 was required for murine pulmonary surfactant synthesis and lung function at birth (PMID: 29083321). It can be detect a band of 30 kDa.

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