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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM111 Polyclonal antibody (24645-1-AP)

    WB analysis using 24645-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24645-1-AP (TMEM111 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TMEM111 Polyclonal antibody (24645-1-AP)

    WB analysis of HEK-293 using 24645-1-AP

    WB result of TMEM111 antibody (24645-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM111 transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TMEM111 Polyclonal antibody (24645-1-AP)

    IHC staining of human ovary cancer using 24645-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24645-1-AP (TMEM111 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TMEM111 Polyclonal antibody (24645-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 24645-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24645-1-AP (TMEM111 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TMEM111 Polyclonal antibody (24645-1-AP)

    IF Staining of A431 using 24645-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TMEM111 antibody (24645-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

TMEM111 Polyclonal antibody

TMEM111 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24645-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EMC3, POB

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HuH-7 cells, MDA-MB-453s cells, NIH/3T3 cells, mouse brain tissue, mouse stomach tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24645-1-AP targets TMEM111 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18236

Product name: Recombinant human TMEM111 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 139-261 aa of BC022807

Sequence: KVPFPLTLRFKPMLQQGIELLTLDASWVSSASWYFLNVFGLRSIYSLILGQDNAADQSRMMQEQMTGAAMAMPADTNKAFKTEWEALELTDHQWALDDVEEELMAKDLHFEGMFKKELQTSIF

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 111
Calculated molecular weight 261 aa, 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC022807
Gene Symbol TMEM111
Gene ID (NCBI) 55831
RRIDAB_3085744
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0I2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM111,also names as EMC3, is part of the endoplasmic reticulum-associated secretory pathway (PMID: 19797678). TMEM111 was required for murine pulmonary surfactant synthesis and lung function at birth (PMID: 29083321). It can be detect a band of 30 kDa.

Protocols

Product Specific Protocols
IF protocol for TMEM111 antibody 24645-1-APDownload protocol
IHC protocol for TMEM111 antibody 24645-1-APDownload protocol
WB protocol for TMEM111 antibody 24645-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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