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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM109 Polyclonal antibody (31438-1-AP)

    WB analysis using 31438-1-AP (same clone as 31438-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31438-1-AP (TMEM109 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM109 Polyclonal antibody (31438-1-AP)

    WB analysis using 31438-1-AP (same clone as 31438-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31438-1-AP (TMEM109 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IHC staining of mouse pancreas using 31438-1-AP (same clone as 31438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 31438-1-AP (TMEM109 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IHC staining of mouse skeletal muscle using 31438-1-AP (same clone as 31438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31438-1-AP (TMEM109 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IHC staining of mouse skeletal muscle using 31438-1-AP (same clone as 31438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31438-1-AP (TMEM109 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IF Staining of mouse skeletal muscle using 31438-1-AP (same clone as 31438-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TMEM109 antibody (31438-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IF Staining of mouse skeletal muscle using 31438-1-AP (same clone as 31438-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TMEM109 antibody (31438-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

TMEM109 Polyclonal antibody, PBS Only

TMEM109 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 31438-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Mg23, Mitsugumin 23, transmembrane protein 109

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31438-1-PBS targets TMEM109 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34866

Product name: Recombinant human TMEM109 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 194-243 aa of BC001309

Sequence: ILYALLSRLTGSRASGAQLEAKVRGLERQVEELRWRQRRAAKGARSVEEE

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 109
Calculated molecular weight 26 kDa
Observed molecular weight22 kDa
GenBank accession numberBC001309
Gene Symbol TMEM109
Gene ID (NCBI) 79073
RRIDAB_3669983
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9BVC6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TMEM109, also known as mg23 or mitsugumin-23, is a transmembrane protein localized to the sarcoplasmic/endoplasmic reticulum and nuclear membranes. TMEM109 is a ball-shaped cation channel in the sarcoplasmic reticulum (SR).

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