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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM109 Polyclonal antibody (31438-1-AP)

    WB analysis using 31438-1-AP (same clone as 31438-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31438-1-AP (TMEM109 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM109 Polyclonal antibody (31438-1-AP)

    WB analysis using 31438-1-AP (same clone as 31438-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31438-1-AP (TMEM109 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IHC staining of mouse pancreas using 31438-1-AP (same clone as 31438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 31438-1-AP (TMEM109 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IHC staining of mouse skeletal muscle using 31438-1-AP (same clone as 31438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31438-1-AP (TMEM109 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IHC staining of mouse skeletal muscle using 31438-1-AP (same clone as 31438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31438-1-AP (TMEM109 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IF Staining of mouse skeletal muscle using 31438-1-AP (same clone as 31438-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TMEM109 antibody (31438-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IF Staining of mouse skeletal muscle using 31438-1-AP (same clone as 31438-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TMEM109 antibody (31438-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31438-1-PBS in a different storage buffer formulation.

TMEM109 Polyclonal antibody, PBS Only

TMEM109 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 31438-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Mg23, Mitsugumin 23, transmembrane protein 109

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31438-1-PBS targets TMEM109 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34866

Product name: Recombinant human TMEM109 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 194-243 aa of BC001309

Sequence: ILYALLSRLTGSRASGAQLEAKVRGLERQVEELRWRQRRAAKGARSVEEE

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 109
Calculated molecular weight 26 kDa
Observed molecular weight22 kDa
GenBank accession numberBC001309
Gene Symbol TMEM109
Gene ID (NCBI) 79073
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9BVC6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TMEM109, also known as mg23 or mitsugumin-23, is a transmembrane protein localized to the sarcoplasmic/endoplasmic reticulum and nuclear membranes. TMEM109 is a ball-shaped cation channel in the sarcoplasmic reticulum (SR).

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