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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM109 Polyclonal antibody (31438-1-AP)

    WB analysis using 31438-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31438-1-AP (TMEM109 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TMEM109 Polyclonal antibody (31438-1-AP)

    WB analysis using 31438-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31438-1-AP (TMEM109 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IHC staining of mouse pancreas using 31438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 31438-1-AP (TMEM109 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IHC staining of mouse skeletal muscle using 31438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31438-1-AP (TMEM109 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IHC staining of mouse skeletal muscle using 31438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31438-1-AP (TMEM109 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IF Staining of mouse skeletal muscle using 31438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TMEM109 antibody (31438-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using TMEM109 Polyclonal antibody (31438-1-AP)

    IF Staining of mouse skeletal muscle using 31438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TMEM109 antibody (31438-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM109 Polyclonal antibody

TMEM109 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 31438-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Mg23, Mitsugumin 23, transmembrane protein 109

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, U-251 cells, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31438-1-AP targets TMEM109 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34866

Product name: Recombinant human TMEM109 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 194-243 aa of BC001309

Sequence: ILYALLSRLTGSRASGAQLEAKVRGLERQVEELRWRQRRAAKGARSVEEE

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 109
Calculated molecular weight 26 kDa
Observed molecular weight22 kDa
GenBank accession numberBC001309
Gene Symbol TMEM109
Gene ID (NCBI) 79073
RRIDAB_3669983
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9BVC6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM109, also known as mg23 or mitsugumin-23, is a transmembrane protein localized to the sarcoplasmic/endoplasmic reticulum and nuclear membranes. TMEM109 is a ball-shaped cation channel in the sarcoplasmic reticulum (SR).

Protocols

Product Specific Protocols
IF protocol for TMEM109 antibody 31438-1-APDownload protocol
IHC protocol for TMEM109 antibody 31438-1-APDownload protocol
WB protocol for TMEM109 antibody 31438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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