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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TIP30 Polyclonal antibody (10251-1-AP)

    WB analysis using 10251-1-AP (same clone as 10251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10251-1-AP (TIP30 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10251-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SW 1990 cells using TIP30 Polyclonal antibody (10251-1-AP)

    WB analysis of SW 1990 using 10251-1-AP (same clone as 10251-1-PBS)

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 10251-1-AP (TIP30 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TIP30 Polyclonal antibody (10251-1-AP)

    IHC staining of human liver using 10251-1-AP (same clone as 10251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10251-1-AP (TIP30 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TIP30 Polyclonal antibody (10251-1-AP)

    IHC staining of human liver using 10251-1-AP (same clone as 10251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10251-1-AP (TIP30 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TIP30 Polyclonal antibody (10251-1-AP)

    IHC staining of human liver cancer using 10251-1-AP (same clone as 10251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10251-1-AP (TIP30 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TIP30 Polyclonal antibody (10251-1-AP)

    IHC staining of human liver cancer using 10251-1-AP (same clone as 10251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10251-1-AP (TIP30 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10251-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TIP30 Polyclonal antibody (10251-1-AP)

    IF Staining of HepG2 using 10251-1-AP (same clone as 10251-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIP30 antibody (10251-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10251-1-PBS in a different storage buffer formulation.

TIP30 Polyclonal antibody, PBS Only

TIP30 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10251-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HTATIP2, 30 kDa HIV-1 TAT-interacting protein, CC3, EC:1.1.1.-, HIV-1 TAT-interactive protein 2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10251-1-PBS targets TIP30 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0353

Product name: Recombinant human TIP30 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 82-276 aa of BC002439

Sequence: TLIGRRKLTFDEEAYKNVNQEVVDFEKLDDYASAFQGHDVGFCCLGTTRGKAGAEGFVRVDRDYVLKSAELAKAGGCKHFNLLSSKGADKSSNFLYLQVKGEVEAKVEELKFDRYSVFRPGVLLCDRQESRPGEWLVRKFFGSLPDSWARGHSVPVVTVVRAMLNNVVRPRDKQMELLENKAIHDLGKAHGSLKP

相同性解析による交差性が予測される生物種
Full Name HIV-1 Tat interactive protein 2, 30kDa
Calculated molecular weight 27 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC002439
Gene Symbol TIP30
Gene ID (NCBI) 10553
RRIDAB_2279817
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUP3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TIP30/CC3 was first identified and characterized as a "candidate" tumor-suppressor gene in 1997. TIP30 is involved in the control of cell apoptosis, growth, metastasis, angiogenesis, DNA repair, and tumor cell metabolism.

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