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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TIP30 Polyclonal antibody (10251-1-AP)

    WB analysis of HeLa using 10251-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10251-1-AP (TIP30 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SW 1990 cells using TIP30 Polyclonal antibody (10251-1-AP)

    WB analysis of SW 1990 using 10251-1-AP

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 10251-1-AP (TIP30 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using TIP30 Polyclonal antibody (10251-1-AP)

    IHC staining of human liver using 10251-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10251-1-AP (TIP30 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TIP30 Polyclonal antibody (10251-1-AP)

    IHC staining of human liver using 10251-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10251-1-AP (TIP30 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TIP30 Polyclonal antibody (10251-1-AP)

    IHC staining of human liver cancer using 10251-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10251-1-AP (TIP30 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TIP30 Polyclonal antibody (10251-1-AP)

    IHC staining of human liver cancer using 10251-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10251-1-AP (TIP30 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TIP30 Polyclonal antibody

TIP30 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10251-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

HTATIP2, 30 kDa HIV-1 TAT-interacting protein, CC3, EC:1.1.1.-, HIV-1 TAT-interactive protein 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, SW 1990 cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10251-1-AP targets TIP30 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0353

Product name: Recombinant human TIP30 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 82-276 aa of BC002439

Sequence: TLIGRRKLTFDEEAYKNVNQEVVDFEKLDDYASAFQGHDVGFCCLGTTRGKAGAEGFVRVDRDYVLKSAELAKAGGCKHFNLLSSKGADKSSNFLYLQVKGEVEAKVEELKFDRYSVFRPGVLLCDRQESRPGEWLVRKFFGSLPDSWARGHSVPVVTVVRAMLNNVVRPRDKQMELLENKAIHDLGKAHGSLKP

相同性解析による交差性が予測される生物種
Full Name HIV-1 Tat interactive protein 2, 30kDa
Calculated molecular weight 27 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC002439
Gene Symbol TIP30
Gene ID (NCBI) 10553
RRIDAB_2279817
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUP3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TIP30/CC3 was first identified and characterized as a "candidate" tumor-suppressor gene in 1997. TIP30 is involved in the control of cell apoptosis, growth, metastasis, angiogenesis, DNA repair, and tumor cell metabolism.

Protocols

Product Specific Protocols
IHC protocol for TIP30 antibody 10251-1-APDownload protocol
WB protocol for TIP30 antibody 10251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

Role of GRK2 in Trophoblast Necroptosis and Spiral Artery Remodeling: Implications for Preeclampsia Pathogenesis.

Authors - Zi Lv
View Article
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