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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using TIMP-4 Polyclonal antibody (12326-1-AP)

    WB analysis of fetal human brain using 12326-1-AP (same clone as 12326-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12326-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using TIMP-4 Polyclonal antibody (12326-1-AP)

    WB analysis of HT-1080 using 12326-1-AP (same clone as 12326-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12326-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using TIMP-4 Polyclonal antibody (12326-1-AP)

    WB analysis of BxPC-3 using 12326-1-AP (same clone as 12326-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12326-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using TIMP-4 Polyclonal antibody (12326-1-AP)

    WB analysis of human heart using 12326-1-AP (same clone as 12326-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TIMP-4 Polyclonal antibody (12326-1-AP)

    IHC staining of human gliomas using 12326-1-AP (same clone as 12326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TIMP-4 Polyclonal antibody (12326-1-AP)

    IHC staining of human gliomas using 12326-1-AP (same clone as 12326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TIMP-4 Polyclonal antibody (12326-1-AP)

    IHC staining of human heart using 12326-1-AP (same clone as 12326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TIMP-4 Polyclonal antibody (12326-1-AP)

    IHC staining of human heart using 12326-1-AP (same clone as 12326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12326-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TIMP-4 Polyclonal antibody (12326-1-AP)

    IF Staining of MCF-7 using 12326-1-AP (same clone as 12326-1-PBS)

    Immunofluorescent analysis of MCF-7 cells using 12326-1-AP (TIMP-4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 12326-1-PBS in a different storage buffer formulation.

TIMP-4 Polyclonal antibody, PBS Only

TIMP-4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12326-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12326-1-PBS targets TIMP-4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2927

Product name: Recombinant human TIMP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-224 aa of BC010553

Sequence: MPGSPRPAPSWVLLLRLLALLRPPGLGEACSCAPAHPQQHICHSALVIRAKISSEKVVPASADPADTEKMLRYEIKQIKMFKGFEKVKDVQYIYTPFDSSLCGVKLEANSQKQYLLTGQVLSDGKVFIHLCNYIEPWEDLSLVQRESLNHHYHLNCGCQITTCYTVPCTISAPNECLWTDWLLERKLYGYQAQHYVCMKHVDGTCSWYRGHLPLRKEFVDIVQP

相同性解析による交差性が予測される生物種
Full Name TIMP metallopeptidase inhibitor 4
Calculated molecular weight 224 aa, 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC010553
Gene Symbol TIMP4
Gene ID (NCBI) 7079
RRIDAB_10638303
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99727
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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