Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of fetal human brain using 12326-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 12326-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 12326-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 12326-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 12326-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human gliomas using 12326-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 12326-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 12326-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 12326-1-AP
Immunofluorescent analysis of MCF-7 cells using 12326-1-AP (TIMP-4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

TIMP-4 Polyclonal antibody

TIMP-4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12326-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TIMP4, TIMP 4, Metalloproteinase inhibitor 4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	fetal human brain tissue, BxPC-3 cells, HT-1080 cells, human heart tissue
Positive IHC detected in	human gliomas tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IF	See 1 publications below

Product Information

12326-1-AP targets TIMP-4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2927 Product name: Recombinant human TIMP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC010553 Sequence: MPGSPRPAPSWVLLLRLLALLRPPGLGEACSCAPAHPQQHICHSALVIRAKISSEKVVPASADPADTEKMLRYEIKQIKMFKGFEKVKDVQYIYTPFDSSLCGVKLEANSQKQYLLTGQVLSDGKVFIHLCNYIEPWEDLSLVQRESLNHHYHLNCGCQITTCYTVPCTISAPNECLWTDWLLERKLYGYQAQHYVCMKHVDGTCSWYRGHLPLRKEFVDIVQP 相同性解析による交差性が予測される生物種
Full Name	TIMP metallopeptidase inhibitor 4
Calculated molecular weight	224 aa, 26 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC010553
Gene Symbol	TIMP4
Gene ID (NCBI)	7079
RRID	AB_10638303
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99727
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for TIMP-4 antibody 12326-1-AP	Download protocol
IHC protocol for TIMP-4 antibody 12326-1-AP	Download protocol
WB protocol for TIMP-4 antibody 12326-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Med Chem Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis. Authors - Ting Peng View Article
mouse	WB,IF	Viruses Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model. Authors - Wangquan Ji View Article
human	WB	J Biochem Mol Toxicol Pacenta polypeptide injection alleviates the fibrosis and inflammation in cigarette smoke extracts-induced BEAS-2B cells by modulating MMP-9/TIMP-1 signaling Authors - Wei Xu View Article
human	WB	Biochem Biophys Res Commun Molecularly targeted anti-cancer drugs inhibit the invasion and metastasis of hepatocellular carcinoma by regulating the expression of MMP and TIMP gene families. Authors - Xiao-Xiao He View Article
mouse	WB	PLoS One Fructose-1,6-bisphosphate prevents pulmonary fibrosis by regulating extracellular matrix deposition and inducing phenotype reversal of lung myofibroblasts. Authors - Henrique Bregolin Dias View Article
human	WB	Biomed Res Int Apatinib Inhibits the Invasion and Metastasis of Liver Cancer Cells by Downregulating MMP-Related Proteins via Regulation of the NF-κB Signaling Pathway. Authors - Xiaoxiao He View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of fetal human brain using 12326-1-AP

WB analysis of human heart using 12326-1-AP

WB analysis of HT-1080 using 12326-1-AP

WB analysis of BxPC-3 using 12326-1-AP

IHC staining of human gliomas using 12326-1-AP

IHC staining of human gliomas using 12326-1-AP

IHC staining of human heart using 12326-1-AP

IHC staining of human heart using 12326-1-AP

IF Staining of MCF-7 using 12326-1-AP

TIMP-4 Polyclonal antibody

TIMP-4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.

Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model.

Pacenta polypeptide injection alleviates the fibrosis and inflammation in cigarette smoke extracts-induced BEAS-2B cells by modulating MMP-9/TIMP-1 signaling

Molecularly targeted anti-cancer drugs inhibit the invasion and metastasis of hepatocellular carcinoma by regulating the expression of MMP and TIMP gene families.

Fructose-1,6-bisphosphate prevents pulmonary fibrosis by regulating extracellular matrix deposition and inducing phenotype reversal of lung myofibroblasts.

Apatinib Inhibits the Invasion and Metastasis of Liver Cancer Cells by Downregulating MMP-Related Proteins via Regulation of the NF-κB Signaling Pathway.