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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THUMPD1 Polyclonal antibody (14921-1-AP)

    WB analysis using 14921-1-AP (same clone as 14921-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using THUMPD1 Polyclonal antibody (14921-1-AP)

    WB analysis using 14921-1-AP (same clone as 14921-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using THUMPD1 Polyclonal antibody (14921-1-AP)

    WB analysis using 14921-1-AP (same clone as 14921-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using THUMPD1 Polyclonal antibody (14921-1-AP)

    IHC staining of human breast cancer using 14921-1-AP (same clone as 14921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using THUMPD1 Polyclonal antibody (14921-1-AP)

    IHC staining of human breast cancer using 14921-1-AP (same clone as 14921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14921-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using THUMPD1 Polyclonal antibody (14921-1-AP)

    IF Staining of MCF-7 using 14921-1-AP (same clone as 14921-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using THUMPD1 antibody (14921-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14921-1-PBS in a different storage buffer formulation.

THUMPD1 Polyclonal antibody, PBS Only

THUMPD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14921-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

THUMP domain-containing protein 1, THUMPD 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14921-1-PBS targets THUMPD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6739

Product name: Recombinant human THUMPD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-353 aa of BC000448

Sequence: MAAPAQQTTQPGGGKRKGKAQYVLAKRARRCDAGGPRQLEPGLQGILITCNMNERKCVEEAYSLLNEYGDDMYGPEKFTDKDQQPSGSEGEDDDAEAALKKEVGDIKASTEMRLRRFQSVESGANNVVFIRTLGIEPEKLVHHILQDMYKTKKKKTRVILRMLPISGTCKAFLEDMKKYAETFLEPWFKAPNKGTFQIVYKSRNNSHVNREEVIRELAGIVCTLNSENKVDLTNPQYTVVVEIIKAVCCLSVVKDYMLFRKYNLQEVVKSPKDPSQLNSKQGNGKEAKLESADKSDQNNTAEGKNNQQVPENTEELGQTKPTSNPQVVNEGGAKPELASQATEGSKSNENDFS

相同性解析による交差性が予測される生物種
Full Name THUMP domain containing 1
Calculated molecular weight 39 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC000448
Gene Symbol THUMPD1
Gene ID (NCBI) 55623
RRIDAB_2271785
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXG2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

THUMPD1, a THUMP domain-containing RNA-binding protein, partners with NAT10 to mediate N4-acetylcytidine (ac4C) modification, enhancing RNA stability, export, and translation. Dysregulated THUMPD1 promotes proliferation, migration, invasion, and EMT.

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