Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells, MCF-7 cells, T-47D cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
14921-1-AP targets THUMPD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6739 Product name: Recombinant human THUMPD1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-353 aa of BC000448 Sequence: MAAPAQQTTQPGGGKRKGKAQYVLAKRARRCDAGGPRQLEPGLQGILITCNMNERKCVEEAYSLLNEYGDDMYGPEKFTDKDQQPSGSEGEDDDAEAALKKEVGDIKASTEMRLRRFQSVESGANNVVFIRTLGIEPEKLVHHILQDMYKTKKKKTRVILRMLPISGTCKAFLEDMKKYAETFLEPWFKAPNKGTFQIVYKSRNNSHVNREEVIRELAGIVCTLNSENKVDLTNPQYTVVVEIIKAVCCLSVVKDYMLFRKYNLQEVVKSPKDPSQLNSKQGNGKEAKLESADKSDQNNTAEGKNNQQVPENTEELGQTKPTSNPQVVNEGGAKPELASQATEGSKSNENDFS 相同性解析による交差性が予測される生物種 |
| Full Name | THUMP domain containing 1 |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC000448 |
| Gene Symbol | THUMPD1 |
| Gene ID (NCBI) | 55623 |
| RRID | AB_2271785 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NXG2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
THUMPD1, a THUMP domain-containing RNA-binding protein, partners with NAT10 to mediate N4-acetylcytidine (ac4C) modification, enhancing RNA stability, export, and translation. Dysregulated THUMPD1 promotes proliferation, migration, invasion, and EMT.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for THUMPD1 antibody 14921-1-AP | Download protocol |
| IHC protocol for THUMPD1 antibody 14921-1-AP | Download protocol |
| IF protocol for THUMPD1 antibody 14921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ Lactylation of NAT10 promotes N4-acetylcytidine modification on tRNASer-CGA-1-1 to boost oncogenic DNA virus KSHV reactivation | ||
Biosci Rep Pan-cancer analysis of N4-acetylcytidine adaptor THUMPD1 as a predictor for prognosis and immunotherapy. | ||
iScience RNA modification-related genes illuminate prognostic signature and mechanism in esophageal squamous cell carcinoma |