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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using THOC2 Polyclonal antibody (55178-1-AP)

    WB analysis of HeLa using 55178-1-AP (same clone as 55178-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using THOC2 Polyclonal antibody (55178-1-AP)

    WB analysis of Jurkat using 55178-1-AP (same clone as 55178-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using THOC2 Polyclonal antibody (55178-1-AP)

    IP experiment of HeLa using 55178-1-AP (same clone as 55178-1-PBS)

    IP result of anti-THOC2 (IP:55178-1-AP, 4ug; Detection:55178-1-AP 1:600) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using THOC2 Polyclonal antibody (55178-1-AP)

    IF Staining of HeLa using 55178-1-AP (same clone as 55178-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC2 antibody (55178-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using THOC2 Polyclonal antibody (55178-1-AP)

    IF Staining of HeLa using 55178-1-AP (same clone as 55178-1-PBS)

    Immunofluorescent analysis of HeLa cells using 55178-1-AP (THOC2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

THOC2 Polyclonal antibody, PBS Only

THOC2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 55178-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55178-1-PBS targets THOC2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name THO complex 2
Calculated molecular weight 183 kDa
Observed molecular weight 150 kDa
GenBank accession numberNM_020449
Gene Symbol THOC2
Gene ID (NCBI) 57187
RRIDAB_2881283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NI27
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

THOC2 also termed as THO complex 2 or CXorf3 is 1593 amino acid protein, which belongs to the THOC2 family. THOC2 is a component of heteromultimeric THO/TREX (transcription/export) complex, which is a multi-protein structure consisting of THOC1, THOC2, THOC3, BAT1 and ALY. The TREX complex associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism.