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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using THOC2 Polyclonal antibody (55178-1-AP)

    WB analysis of HeLa using 55178-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using THOC2 Polyclonal antibody (55178-1-AP)

    WB analysis using 55178-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using THOC2 Polyclonal antibody (55178-1-AP)

    WB analysis of Jurkat using 55178-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using THOC2 Polyclonal antibody (55178-1-AP)

    IP experiment of HeLa using 55178-1-AP

    IP result of anti-THOC2 (IP:55178-1-AP, 4ug; Detection:55178-1-AP 1:600) with HeLa cells lysate 1600 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using THOC2 Polyclonal antibody (55178-1-AP)

    IF Staining of HeLa using 55178-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC2 antibody (55178-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using THOC2 Polyclonal antibody (55178-1-AP)

    IF Staining of HeLa using 55178-1-AP

    Immunofluorescent analysis of HeLa cells using 55178-1-AP (THOC2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

  • Featured Product
  • KD/KO Validated

THOC2 Polyclonal antibody

THOC2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 55178-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

CXorf3, hTREX120, THO complex 2, THO complex subunit 2, Tho2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55178-1-AP targets THOC2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name THO complex 2
Calculated molecular weight 183 kDa
Observed molecular weight 150 kDa
GenBank accession numberNM_020449
Gene Symbol THOC2
Gene ID (NCBI) 57187
RRIDAB_2881283
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NI27
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

THOC2 also termed as THO complex 2 or CXorf3 is 1593 amino acid protein, which belongs to the THOC2 family. THOC2 is a component of heteromultimeric THO/TREX (transcription/export) complex, which is a multi-protein structure consisting of THOC1, THOC2, THOC3, BAT1 and ALY. The TREX complex associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism.

Protocols

Product Specific Protocols
IF protocol for THOC2 antibody 55178-1-APDownload protocol
IP protocol for THOC2 antibody 55178-1-APDownload protocol
WB protocol for THOC2 antibody 55178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Clinical and functional characterization of recurrent missense variants implicated in THOC6-related intellectual disability.

Authors - Mattioli Francesca
View Article
humanWB

Viruses

Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.

Authors - Toshie Sakuma
  • KD Validated
View Article
humanWB

Mol Cell

YTHDC1 cooperates with the THO complex to prevent RNA-damage-induced DNA breaks

Authors - Ning Tsao
View Article
humanWB

Int J Biol Macromol

Targeting mRNA export complex macromolecules THO subunits (Thoc2 and Thoc5) for somatic cell reprograming

Authors - Abdur Rehman
View Article
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