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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated THOC1 Polyclonal antibody

THOC1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10920

Synonyms

HPR1, hTREX84, Nuclear matrix protein p84, P84, P84N5, THO complex 1, THO complex subunit 1, Tho1, THOC1

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-10920

    FC experiment of HeLa using CL488-10920

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human THOC1 (CL488-10920) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy THOC1 Ready-To-Use IHC Kit. THOC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10920-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-10920 targets THOC1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THOC1 fusion protein Ag1352 相同性解析による交差性が予測される生物種
Full Name THO complex 1
Calculated molecular weight 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC010381
Gene symbol THOC1
Gene ID (NCBI) 9984
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

THOC1 (THO complex subunit 1), also known as Tho1, P84, HPR1 or P84N5, is a 657 amino acid nuclear matrix protein and is evolutionarily conserved from yeast to humans. THOC1 contains one death domain and is a component of the heteromultimeric THO/TREX (transcription/export) complex along with THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels along with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. THOC1 is expressed at high levels in breast cancer cells and at relatively low levels in normal epithelia. A reduction of THOC1 in cancer cell lines results in promoted cancer cell apoptosis and reduced cell proliferation. This suggests that cancer cells are dependent on the high levels of THOC1 expression and therefore THOC1 may be a good target for cancer therapy. This antibody recognizes the 84kd THOC1 protein.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 THOC1 antibody CL488-10920Download protocol
Standard Protocols
Click here to view our Standard Protocols