Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis of human stomach using 13681-1-AP (same clone as 13681-1-PBS)
human stomach tissue were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
WB analysis using 13681-1-AP (same clone as 13681-1-PBS)
Human urine tissue were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
WB analysis of human stomach using 13681-1-AP (same clone as 13681-1-PBS)
human stomach sample were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
IP experiment of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)
IP result of anti-TFF2 (IP:13681-1-AP, 4ug; Detection:13681-1-AP 1:600) with mouse stomach tissue lysate 4000ug. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 13681-1-AP (same clone as 13681-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13681-1-AP (TFF2 Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 13681-1-AP (same clone as 13681-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13681-1-AP (TFF2 Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
IHC staining of mouse small intestine using 13681-1-AP (same clone as 13681-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
IHC staining of mouse small intestine using 13681-1-AP (same clone as 13681-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 13681-1-AP (same clone as 13681-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13681-1-AP (TFF2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 13681-1-AP (same clone as 13681-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13681-1-AP (TFF2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.
IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

TFF2 Polyclonal antibody, PBS Only

TFF2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Cat No. 13681-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

SML1, SP, Spasmolysin, Spasmolytic polypeptide, trefoil factor 2

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13681-1-PBS targets TFF2 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4537 Product name: Recombinant human TFF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-129 aa of BC032820 Sequence: MGRRDAQLLAALLVLGLCALAGSEKPSPCQCSRLSPHNRTNCGFPGITSDQCFDNGCCFDSSVTGVPWCFHPLPKQESDQCVMEVSDRRNCGYPGISPEECASRKCCFSNFIFEVPWCFFPKSVEDCHY 相同性解析による交差性が予測される生物種
Full Name	trefoil factor 2
Calculated molecular weight	129 aa, 14 kDa
Observed molecular weight	18-20 kDa
GenBank accession number	BC032820
Gene Symbol	TFF2
Gene ID (NCBI)	7032
RRID	AB_10598321
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q03403
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

WB analysis using 13681-1-AP (same clone as 13681-1-PBS)

WB analysis of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

IP experiment of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

IHC staining of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

IHC staining of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

IHC staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

IHC staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

IHC staining of mouse small intestine using 13681-1-AP (same clone as 13681-1-PBS)

IHC staining of mouse small intestine using 13681-1-AP (same clone as 13681-1-PBS)

IHC staining of human stomach cancer using 13681-1-AP (same clone as 13681-1-PBS)

IHC staining of human stomach cancer using 13681-1-AP (same clone as 13681-1-PBS)

IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

TFF2 Polyclonal antibody, PBS Only

TFF2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

Able^™