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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TFAP2E Polyclonal antibody

TFAP2E Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25829-1-AP

Synonyms

AP2 epsilon, AP2E, TFAP2E

Validation Data Gallery

Applications:
  • WB analysis of A375 using 25829-1-AP

    WB analysis of A375 using 25829-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 25829-1-AP (TFAP2E Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 25829-1-AP

    WB analysis of rat brain using 25829-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25829-1-AP (TFAP2E Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 25829-1-AP

    WB analysis of A375 using 25829-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 25829-1-AP (TFAP2E Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 25829-1-AP

    WB analysis of mouse brain using 25829-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25829-1-AP (TFAP2E Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 25829-1-AP

    IHC staining of human skin cancer using 25829-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25829-1-AP (TFAP2E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 25829-1-AP

    IHC staining of human skin cancer using 25829-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25829-1-AP (TFAP2E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A375 using 25829-1-AP

    IF Staining of A375 using 25829-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 25829-1-AP (TFAP2E antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 25829-1-AP is TFAP2E Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFAP2E fusion protein Ag23023 相同性解析による交差性が予測される生物種
Full Name transcription factor AP-2 epsilon (activating enhancer binding protein 2 epsilon)
Calculated molecular weight 442 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC041175
Gene symbol TFAP2E
Gene ID (NCBI) 339488
RRIDAB_2880258
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TFAP2E antibody 25829-1-APDownload protocol
IHC protocol for TFAP2E antibody 25829-1-APDownload protocol
IF protocol for TFAP2E antibody 25829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Elife

Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons

Authors - Jennifer M Lin
View Article