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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TFAP2B Polyclonal antibody (13183-1-AP)

    WB analysis of MCF-7 using 13183-1-AP (same clone as 13183-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13183-1-AP (TFAP2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13183-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using TFAP2B Polyclonal antibody (13183-1-AP)

    WB analysis of MCF-7 using 13183-1-AP (same clone as 13183-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13183-1-AP (TFAP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13183-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using TFAP2B Polyclonal antibody (13183-1-AP)

    IP experiment of MCF-7 using 13183-1-AP (same clone as 13183-1-PBS)

    IP result of anti-TFAP2B (IP:13183-1-AP, 4ug; Detection:13183-1-AP 1:500) with MCF-7 cells lysate 800ug. This data was developed using the same antibody clone with 13183-1-PBS in a different storage buffer formulation.

TFAP2B Polyclonal antibody, PBS Only

TFAP2B Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 13183-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13183-1-PBS targets TFAP2B in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3853

Product name: Recombinant human TFAP2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 111-460 aa of BC037225

Sequence: RQEVGSEAGSLLPQPRAALPQLSGLDPRRDYHSVRRPDVLLHSAHHGLDAGMGDSLSLHGLGHPGMEDVQSVEDANNSGMNLLDQSVIKKVPVPPKSVTSLMMNKDGFLGGMSVNTGEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLRERLEKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYICETEFPAKAVSEYLNRQHTDPSDLHSRKNMLLATKQLCKEFTDLLAQDRTPIGNSRPSPILEPGIQSCLTHFSLITHGFGAPAICAALTALQNYLTEALKGMDKMFLNNTTTNRHTSGEGPGSKTGDKEEKHRK

相同性解析による交差性が予測される生物種
Full Name transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
Calculated molecular weight 449 aa, 49 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC037225
Gene Symbol TFAP2B
Gene ID (NCBI) 7021
RRIDAB_2286992
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92481
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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