×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using TFAP2B Polyclonal antibody (13183-1-AP)

    WB analysis of MCF-7 using 13183-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13183-1-AP (TFAP2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TFAP2B Polyclonal antibody (13183-1-AP)

    WB analysis of MCF-7 using 13183-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13183-1-AP (TFAP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using TFAP2B Polyclonal antibody (13183-1-AP)

    IP experiment of MCF-7 using 13183-1-AP

    IP result of anti-TFAP2B (IP:13183-1-AP, 4ug; Detection:13183-1-AP 1:500) with MCF-7 cells lysate 800ug.

TFAP2B Polyclonal antibody

TFAP2B Polyclonal Antibody for WB, IP, ELISA
Cat No. 13183-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

AP 2B, AP2 B, AP2 beta, TFAP2B, Transcription factor AP 2 beta

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IP detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13183-1-AP targets TFAP2B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3853

Product name: Recombinant human TFAP2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 111-460 aa of BC037225

Sequence: RQEVGSEAGSLLPQPRAALPQLSGLDPRRDYHSVRRPDVLLHSAHHGLDAGMGDSLSLHGLGHPGMEDVQSVEDANNSGMNLLDQSVIKKVPVPPKSVTSLMMNKDGFLGGMSVNTGEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLRERLEKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYICETEFPAKAVSEYLNRQHTDPSDLHSRKNMLLATKQLCKEFTDLLAQDRTPIGNSRPSPILEPGIQSCLTHFSLITHGFGAPAICAALTALQNYLTEALKGMDKMFLNNTTTNRHTSGEGPGSKTGDKEEKHRK

相同性解析による交差性が予測される生物種
Full Name transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
Calculated molecular weight 449 aa, 49 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC037225
Gene Symbol TFAP2B
Gene ID (NCBI) 7021
RRIDAB_2286992
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92481
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IP protocol for TFAP2B antibody 13183-1-APDownload protocol
WB protocol for TFAP2B antibody 13183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Front Cell Dev Biol

Transcription factors ETV4 and ETV5 are required for nephron progenitor cell maintenance, distal nephron development and connection to the collecting system.

Authors - Kaitlin Day
View Article
humanWB

Oncol Lett

miR-142-5p promotes renal cell tumorigenesis by targeting TFAP2B.

Authors - Maoshu Zhu
View Article
||
New chat

Able™

正在加载，请稍候...