Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-13359 targets TDRD3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TDRD3 fusion protein Ag4174 相同性解析による交差性が予測される生物種 |
| Full Name | tudor domain containing 3 |
| Calculated molecular weight | 651 aa, 73 kDa |
| Observed molecular weight | 73 kDa, 83 kDa |
| GenBank accession number | BC030514 |
| Gene Symbol | TDRD3 |
| Gene ID (NCBI) | 81550 |
| RRID | AB_3672562 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H7E2 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TDRD3 (Tudor domain-containing protein 3) contains tudor domain which is an approximately 60-amino acid structure motif. TDRD3 functions as a scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. TDRD3 is a transcriptional coactivator that promotes transcription by binding methylarginine marks on histone tails. TDRD3 also possesses an oligosaccharide/nucleotide binding fold and an ubiquitin associated domain capable of binding tetra-ubiquitin, and it is reported to associate with polyribosomes in HeLa cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TDRD3 antibody CL488-13359 | Download protocol |
| FC protocol for CL Plus 488 TDRD3 antibody CL488-13359 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |