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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCIRG1 Polyclonal antibody (12649-1-AP)

    WB analysis using 12649-1-AP (same clone as 12649-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12649-1-AP (TCIRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using TCIRG1 Polyclonal antibody (12649-1-AP)

    WB analysis of A431 using 12649-1-AP (same clone as 12649-1-PBS)

    WB result of TCIRG1 antibody (12649-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCIRG1 transfected A431 cells. This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using TCIRG1 Polyclonal antibody (12649-1-AP)

    WB analysis of U-937 using 12649-1-AP (same clone as 12649-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 12649-1-AP (TCIRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using TCIRG1 Polyclonal antibody (12649-1-AP)

    IHC staining of human stomach using 12649-1-AP (same clone as 12649-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12649-1-AP (TCIRG1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TCIRG1 Polyclonal antibody (12649-1-AP)

    IF Staining of HepG2 using 12649-1-AP (same clone as 12649-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using TCIRG1 antibody 12649-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TCIRG1 Polyclonal antibody, PBS Only

TCIRG1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12649-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

T-cell immune regulator 1, OPTB1, OC-116 kDa, OC116, ATP6V0A3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12649-1-PBS targets TCIRG1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3378

Product name: Recombinant human TCIRG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-395 aa of BC018133

Sequence: CVSRLGELGLVEFRDLNASVSAFQRRFVVDVRRCEELEKTFTFLQEEVRRAGLVLPPPKGRLPAPPPRDLLRIQEETERLAQELRDVRGNQQALRAQLHQLQLHAAVLRQGHEPQLAAAHTDGASERTPLLQAPGGPHQDLRVNFVAGAVEPHKAPALERLLWRACRGFLIASFRELEQPLEHPVTGEPATWMTFLISYWGEQIGQKIRKITDCFHCHVFPFLQQEEARLGALQQLQQQSQELQEVLGETERFLSQVLGRVLQLLPPGQVQVHKMKAVYLALNQCSVSTTHKCLIAEAWCSVRDLPALQEALRDSSMEEGVSAVAHRIPCRDMPPTLIRTNRFTASFQGIVDAYGVGRYQEVNPAPYTIIT

相同性解析による交差性が予測される生物種
Full Name T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
Calculated molecular weight 830 aa, 93 kDa
Observed molecular weight 92 kDa, 65-70 kDa
GenBank accession numberBC018133
Gene Symbol TCIRG1
Gene ID (NCBI) 10312
RRIDAB_10644329
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13488
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TCIRG1, also known as V ATPase 116 kDa isoform a3, TIRC7, OPTB1, belongs to the V-ATPase 116 kDa subunit family, TCIRG1 seems to be directly involved in T-cell activation (PubMed:10329006). Alternative splicing results in multiple transcript variants. Isoform long is highly expressed in osteoclastomas. Isoform short is highly expressed in thymus (PMID: 15809087, 24753205). Mutations in TCIRG1 are associated with Osteopetrosis, autosomal recessive 1 (OPTB1)( PMID: 11532986).

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