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  • KD/KO Validated

TCIRG1 Polyclonal antibody

TCIRG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12649-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

T-cell immune regulator 1, OPTB1, OC-116 kDa, OC116, ATP6V0A3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHL-60 cells, U-937 cells, A431 cells, HuH-7 cells, SKOV-3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12649-1-AP targets TCIRG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCIRG1 fusion protein Ag3378 相同性解析による交差性が予測される生物種
Full Name T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
Calculated molecular weight 830 aa, 93 kDa
Observed molecular weight 92 kDa, 65-70 kDa
GenBank accession numberBC018133
Gene Symbol TCIRG1
Gene ID (NCBI) 10312
RRIDAB_10644329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13488
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TCIRG1, also known as V ATPase 116 kDa isoform a3, TIRC7, OPTB1, belongs to the V-ATPase 116 kDa subunit family, TCIRG1 seems to be directly involved in T-cell activation (PubMed:10329006). Alternative splicing results in multiple transcript variants. Isoform long is highly expressed in osteoclastomas. Isoform short is highly expressed in thymus (PMID: 15809087, 24753205). Mutations in TCIRG1 are associated with Osteopetrosis, autosomal recessive 1 (OPTB1)( PMID: 11532986).

Protocols

Product Specific Protocols
WB protocol for TCIRG1 antibody 12649-1-APDownload protocol
IHC protocol for TCIRG1 antibody 12649-1-APDownload protocol
IF protocol for TCIRG1 antibody 12649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncotarget

PMID: 28978047

Authors - Enrica Balza
mouseIF

Cancer Med

Therapeutic efficacy of proton transport inhibitors alone or in combination with cisplatin in triple negative and hormone sensitive breast cancer models.

Authors - Enrica Balza
humanWB

Oncol Rep

TIRC7 inhibits Th1 cells by upregulating the expression of CTLA‑4 and STAT3 in mice with acute graft‑versus‑host disease.

Authors - Feng Zhu
  • KD Validated
pigWB

Front Microbiol

Lactobacillus gasseri LA39 Activates the Oxidative Phosphorylation Pathway in Porcine Intestinal Epithelial Cells.

Authors - Jun Hu
humanIHC,WB

Cancers (Basel)

Integrative Analysis Identifies TCIRG1 as a Potential Prognostic and Immunotherapy-Relevant Biomarker Associated with Malignant Cell Migration in Clear Cell Renal Cell Carcinoma

Authors - Chao Xu
  • KD Validated
humanWB

Cancer Cell Int

Glycolysis-related biomarker TCIRG1 participates in regulation of renal cell carcinoma progression and tumor immune microenvironment by affecting aerobic glycolysis and AKT/mTOR signaling pathway

Authors - Sichen Di
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