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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using TCB1 Recombinant antibody (84878-5-RR)

    WB analysis of mouse thymus using 84878-5-RR (same clone as 84878-5-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 84878-5-RR (TCB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84878-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TCB1 Recombinant antibody (84878-5-RR)

    WB analysis using 84878-5-RR (same clone as 84878-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84878-5-RR (TCB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84878-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using TCB1 Recombinant antibody (84878-5-RR)

    IHC staining of mouse spleen using 84878-5-RR (same clone as 84878-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84878-5-RR (TCB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84878-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using TCB1 Recombinant antibody (84878-5-RR)

    IHC staining of mouse spleen using 84878-5-RR (same clone as 84878-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84878-5-RR (TCB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84878-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using TCB1 Recombinant antibody (84878-5-RR)

    IHC staining of mouse thymus using 84878-5-RR (same clone as 84878-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84878-5-RR (TCB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84878-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using TCB1 Recombinant antibody (84878-5-RR)

    IHC staining of mouse thymus using 84878-5-RR (same clone as 84878-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84878-5-RR (TCB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84878-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse thymus tissue using TCB1 Recombinant antibody (84878-5-RR)

    IF Staining of mouse thymus using 84878-5-RR (same clone as 84878-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using TCB1 antibody (84878-5-RR, Clone: 241802B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84878-5-PBS in a different storage buffer formulation.

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Mouse TCB1 Recombinant antibody, PBS Only

TCB1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84878-5-PBS
Clone No.241802B12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

241802B12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84878-5-PBS targets TCB1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TCB1 fusion protein Eg2437 相同性解析による交差性が予測される生物種
Full Name TCB1
Calculated molecular weight19 kDa
Observed molecular weight38 kDa
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01852
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.