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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using TBC1D7 Polyclonal antibody (20769-1-AP)

    WB analysis of human placenta using 20769-1-AP (same clone as 20769-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 20769-1-AP (TBC1D7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20769-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TBC1D7 Polyclonal antibody (20769-1-AP)

    WB analysis using 20769-1-AP (same clone as 20769-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20769-1-AP (TBC1D7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20769-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Neuro-2a cells using TBC1D7 Polyclonal antibody (20769-1-AP)

    IP experiment of Neuro-2a using 20769-1-AP (same clone as 20769-1-PBS)

    IP result of anti-TBC1D7 (IP:20769-1-AP, 4ug; Detection:20769-1-AP 1:500) with Neuro-2a cells lysate 1440 ug. This data was developed using the same antibody clone with 20769-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using TBC1D7 Polyclonal antibody (20769-1-AP)

    IP experiment of HeLa using 20769-1-AP (same clone as 20769-1-PBS)

    IP result of anti-TBC1D7 (IP:20769-1-AP, 4ug; Detection:20769-1-AP 1:500) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 20769-1-PBS in a different storage buffer formulation.

TBC1D7 Polyclonal antibody, PBS Only

TBC1D7 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 20769-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

PIG51, TBC1 domain family member 7, TBC1 domain family, member 7, TBC7

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20769-1-PBS targets TBC1D7 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14756

Product name: Recombinant human TBC1D7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-293 aa of BC007054

Sequence: MTEDSQRNFRSVYYEKVGFRGVEEKKSLEILLKDDRLDTEKLCTFSQRFPLPSMYRALVWKVLLGILPPHHESHAKVMMYRKEQYLDVLHALKVVRFVSDATPQAEVYLRMYQLESGKLPRSPSFPLEPDDEVFLAIAKAMEEMVEDSVDCYWITRRFVNQLNTKYRDSLPQLPKAFEQYLNLEDGRLLTHLRMCSAAPKLPYDLWFKRCFAGCLPESSLQRVWDKVVSGSCKILVFVAVEILLTFKIKVMALNSAEKITKFLENIPQDSSDAIVSKAIDLWHKHCGTPVHSS

相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 7
Calculated molecular weight 293 aa, 34 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC007054
Gene Symbol TBC1D7
Gene ID (NCBI) 51256
RRIDAB_3721768
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0N9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TBC1D7 is a member of the TBC-domain containing protein family. The encoded protein functions as a subunit of the tuberous sclerosis TSC1-TSC2 complex which plays a role in the regulation of cellular growth and differentiation. Mutations in this gene have been associated with autosomal recessive megalencephaly.

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