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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using TBC1D7 Polyclonal antibody (20769-1-AP)

    WB analysis of human placenta using 20769-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 20769-1-AP (TBC1D7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TBC1D7 Polyclonal antibody (20769-1-AP)

    WB analysis using 20769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20769-1-AP (TBC1D7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Neuro-2a cells using TBC1D7 Polyclonal antibody (20769-1-AP)

    IP experiment of Neuro-2a using 20769-1-AP

    IP result of anti-TBC1D7 (IP:20769-1-AP, 4ug; Detection:20769-1-AP 1:500) with Neuro-2a cells lysate 1440 ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using TBC1D7 Polyclonal antibody (20769-1-AP)

    IP experiment of HeLa using 20769-1-AP

    IP result of anti-TBC1D7 (IP:20769-1-AP, 4ug; Detection:20769-1-AP 1:500) with HeLa cells lysate 1200 ug.

TBC1D7 Polyclonal antibody

TBC1D7 Polyclonal Antibody for WB, IP, ELISA
Cat No. 20769-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

PIG51, TBC1 domain family member 7, TBC1 domain family, member 7, TBC7

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRamos cells, human placenta tissue, U266 cells, Neuro-2a cells
Positive IP detected inNeuro-2a cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20769-1-AP targets TBC1D7 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14756

Product name: Recombinant human TBC1D7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-293 aa of BC007054

Sequence: MTEDSQRNFRSVYYEKVGFRGVEEKKSLEILLKDDRLDTEKLCTFSQRFPLPSMYRALVWKVLLGILPPHHESHAKVMMYRKEQYLDVLHALKVVRFVSDATPQAEVYLRMYQLESGKLPRSPSFPLEPDDEVFLAIAKAMEEMVEDSVDCYWITRRFVNQLNTKYRDSLPQLPKAFEQYLNLEDGRLLTHLRMCSAAPKLPYDLWFKRCFAGCLPESSLQRVWDKVVSGSCKILVFVAVEILLTFKIKVMALNSAEKITKFLENIPQDSSDAIVSKAIDLWHKHCGTPVHSS

相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 7
Calculated molecular weight 293 aa, 34 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC007054
Gene Symbol TBC1D7
Gene ID (NCBI) 51256
RRIDAB_3721768
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0N9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBC1D7 is a member of the TBC-domain containing protein family. The encoded protein functions as a subunit of the tuberous sclerosis TSC1-TSC2 complex which plays a role in the regulation of cellular growth and differentiation. Mutations in this gene have been associated with autosomal recessive megalencephaly.

Protocols

Product Specific Protocols
IP protocol for TBC1D7 antibody 20769-1-APDownload protocol
WB protocol for TBC1D7 antibody 20769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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