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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D2B Polyclonal antibody (24462-1-AP)

    WB analysis using 24462-1-AP (same clone as 24462-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24462-1-AP (TBC1D2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24462-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using TBC1D2B Polyclonal antibody (24462-1-AP)

    WB analysis of U-251 using 24462-1-AP (same clone as 24462-1-PBS)

    WB result of TBC1D2B antibody (24462-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D2B transfected U-251 cells. This data was developed using the same antibody clone with 24462-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of BxPC-3 cells using TBC1D2B Polyclonal antibody (24462-1-AP)

    IP experiment of BxPC-3 using 24462-1-AP (same clone as 24462-1-PBS)

    IP result of anti-TBC1D2B (IP:24462-1-AP, 4ug; Detection:24462-1-AP 1:600) with BxPC-3 cells lysate 1200 ug. This data was developed using the same antibody clone with 24462-1-PBS in a different storage buffer formulation.

TBC1D2B Polyclonal antibody, PBS Only

TBC1D2B Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 24462-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

TBC1 domain family member 2B, TBC1 domain family, member 2B

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24462-1-PBS targets TBC1D2B in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19632

Product name: Recombinant human TBC1D2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 324-655 aa of BC152465

Sequence: KDEVIIKLSEGEGNGPPPTVAPSSPSVVPVARDQLELDRLKDNLQGYKTQNKFLNKEILELSALRRNAERRERDLMAKYSSLEAKLCQIESKYLILLQEMKTPVCSEDQGPTREVIAQLLEDALQVESQEQPEQAFVKPHLVSEYDIYGFRTVPEDDEEEKLVAKVRALDLKTLYLTENQEVSTGVKWENYFASTVNREMMCSPELKNLIRAGIPHEHRSKVWKWCVDRHTRKFKDNTEPGHFQTLLQKALEKQNPASKQIELDLLRTLPNNKHYSCPTSEGIQKLRNVLLAFSWRNPDIGYCQGLNRLVAVALLYLEQEDAFWCLVTIVEV

相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 2B
Calculated molecular weight 963 aa, 110 kDa
Observed molecular weight 120-125 kDa
GenBank accession numberBC152465
Gene Symbol TBC1D2B
Gene ID (NCBI) 23102
RRIDAB_3742115
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPU7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TBC1 domain family member 2B (TBC1D2B) contains a functional canonical LIR motif and acts at an early stage of autophagy by binding to both LC3/GABARAP and ATG12 conjugation complexes (PMID: 38226533). When there is a high protein level of RAB22A on early endosome, TBC1D2B is recruited and acts as a brake to prevent early endosomes from maturing into late endosomes (PMID: 39051763).

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