Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, U-251 cells, HL-60 cells, mouse spleen tissue |
| Positive IP detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24462-1-AP targets TBC1D2B in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19632 Product name: Recombinant human TBC1D2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 324-655 aa of BC152465 Sequence: KDEVIIKLSEGEGNGPPPTVAPSSPSVVPVARDQLELDRLKDNLQGYKTQNKFLNKEILELSALRRNAERRERDLMAKYSSLEAKLCQIESKYLILLQEMKTPVCSEDQGPTREVIAQLLEDALQVESQEQPEQAFVKPHLVSEYDIYGFRTVPEDDEEEKLVAKVRALDLKTLYLTENQEVSTGVKWENYFASTVNREMMCSPELKNLIRAGIPHEHRSKVWKWCVDRHTRKFKDNTEPGHFQTLLQKALEKQNPASKQIELDLLRTLPNNKHYSCPTSEGIQKLRNVLLAFSWRNPDIGYCQGLNRLVAVALLYLEQEDAFWCLVTIVEV 相同性解析による交差性が予測される生物種 |
| Full Name | TBC1 domain family, member 2B |
| Calculated molecular weight | 963 aa, 110 kDa |
| Observed molecular weight | 120-125 kDa |
| GenBank accession number | BC152465 |
| Gene Symbol | TBC1D2B |
| Gene ID (NCBI) | 23102 |
| RRID | AB_3742115 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPU7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TBC1 domain family member 2B (TBC1D2B) contains a functional canonical LIR motif and acts at an early stage of autophagy by binding to both LC3/GABARAP and ATG12 conjugation complexes (PMID: 38226533). When there is a high protein level of RAB22A on early endosome, TBC1D2B is recruited and acts as a brake to prevent early endosomes from maturing into late endosomes (PMID: 39051763).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for TBC1D2B antibody 24462-1-AP | Download protocol |
| WB protocol for TBC1D2B antibody 24462-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |