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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D20 Polyclonal antibody (16464-1-AP)

    WB analysis using 16464-1-AP (same clone as 16464-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16464-1-AP (TBC1D20 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16464-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TBC1D20 Polyclonal antibody (16464-1-AP)

    WB analysis using 16464-1-AP (same clone as 16464-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16464-1-AP (TBC1D20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16464-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using TBC1D20 Polyclonal antibody (16464-1-AP)

    WB analysis of A549 using 16464-1-AP (same clone as 16464-1-PBS)

    WB result of TBC1D20 antibody (16464-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D20 transfected A549 cells. This data was developed using the same antibody clone with 16464-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using TBC1D20 Polyclonal antibody (16464-1-AP)

    WB analysis of A549 using 16464-1-AP (same clone as 16464-1-PBS)

    WB result of TBC1D20 antibody (16464-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D20 transfected A549 cells. This data was developed using the same antibody clone with 16464-1-PBS in a different storage buffer formulation.

TBC1D20 Polyclonal antibody, PBS Only

TBC1D20 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 16464-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16464-1-PBS targets TBC1D20 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9539

Product name: Recombinant human TBC1D20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-366 aa of BC014983

Sequence: MALRSAQGDGPTSGHWDGGAEKADFNAKRKKKVAEIHQALNSDPTDVAALRRMAISEGGLLTDEIRRKVWPKLLNVNANDPPPISGKNLRQMSKDYQQVLLDVRRSLRRFPPGMPEEQREGLQEELIDIILLILERNPQLHYYQGYHDIVVTFLLVVGERLATSLVEKLSTHHLRDFMDPTMDNTKHILNYLMPIIDQVNPELHDFMQSAEVGTIFALSWLITWFGHVLSDFRHVVRLYDFFLACHPLMPIYFAAVIVLYREQEVLDCDCDMASVHHLLSQIPQDLPYETLISRAGDLFVQFPPSELAREAAAQQQAERTAASTFKDFELASAQQRPDMVLRQRFRGLLRPEDRTKDVLTKPRTNR

相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 20
Calculated molecular weight 403 aa, 46 kDa
Observed molecular weight38-40 kDa
GenBank accession numberBC014983
Gene Symbol TBC1D20
Gene ID (NCBI) 128637
RRIDAB_3669245
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BZ9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TBC1D20 belongs to a family of GTPase activator proteins of Rab-like small GTPases.TBC1D20 is an endoplasmic reticulum (ER) type II membrane protein with the TBC domain positioned in the cytosol. Depletion of this protein inhibits replication of the virus and HCV infection. Mutations in this gene are associated with Warburg micro syndrome 4.

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