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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D20 Polyclonal antibody (16464-1-AP)

    WB analysis using 16464-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16464-1-AP (TBC1D20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TBC1D20 Polyclonal antibody (16464-1-AP)

    WB analysis using 16464-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16464-1-AP (TBC1D20 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TBC1D20 Polyclonal antibody (16464-1-AP)

    WB analysis of A549 using 16464-1-AP

    WB result of TBC1D20 antibody (16464-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D20 transfected A549 cells.

  • Western Blot (WB) analysis of A549 cells using TBC1D20 Polyclonal antibody (16464-1-AP)

    WB analysis of A549 using 16464-1-AP

    WB result of TBC1D20 antibody (16464-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D20 transfected A549 cells.

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TBC1D20 Polyclonal antibody

TBC1D20 Polyclonal Antibody for WB, ELISA
Cat No. 16464-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C20orf140, TBC1 domain family member 20, TBC1 domain family, member 20, TBC1D20

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHepG2 cells, A549 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16464-1-AP targets TBC1D20 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBC1D20 fusion protein Ag9539 相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 20
Calculated molecular weight 403 aa, 46 kDa
Observed molecular weight38-40 kDa
GenBank accession numberBC014983
Gene Symbol TBC1D20
Gene ID (NCBI) 128637
RRIDAB_3669245
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BZ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBC1D20 belongs to a family of GTPase activator proteins of Rab-like small GTPases.TBC1D20 is an endoplasmic reticulum (ER) type II membrane protein with the TBC domain positioned in the cytosol. Depletion of this protein inhibits replication of the virus and HCV infection. Mutations in this gene are associated with Warburg micro syndrome 4.

Protocols

Product Specific Protocols
WB protocol for TBC1D20 antibody 16464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols