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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TARS2 Polyclonal antibody (15067-1-AP)

    WB analysis of HepG2 using 15067-1-AP (same clone as 15067-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15067-1-AP (TARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TARS2 Polyclonal antibody (15067-1-AP)

    WB analysis of HeLa using 15067-1-AP (same clone as 15067-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15067-1-AP (TARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using TARS2 Polyclonal antibody (15067-1-AP)

    WB analysis of mouse liver using 15067-1-AP (same clone as 15067-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15067-1-AP (TARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15067-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using TARS2 Polyclonal antibody (15067-1-AP)

    IP experiment of mouse liver using 15067-1-AP (same clone as 15067-1-PBS)

    IP result of anti-TARS2 (IP:15067-1-AP, 4ug; Detection:15067-1-AP 1:600) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 15067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TARS2 Polyclonal antibody (15067-1-AP)

    IHC staining of mouse heart using 15067-1-AP (same clone as 15067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TARS2 Polyclonal antibody (15067-1-AP)

    IHC staining of mouse heart using 15067-1-AP (same clone as 15067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TARS2 Polyclonal antibody (15067-1-AP)

    IHC staining of mouse liver using 15067-1-AP (same clone as 15067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TARS2 Polyclonal antibody (15067-1-AP)

    IHC staining of mouse liver using 15067-1-AP (same clone as 15067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15067-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TARS2 Polyclonal antibody (15067-1-AP)

    IF Staining of MCF-7 using 15067-1-AP (same clone as 15067-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TARS2 antibody (15067-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 15067-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TARS2 Polyclonal antibody, PBS Only

TARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15067-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:6.1.1.3, TARSL1, Threonine  tRNA ligase, Threonine--tRNA ligase, mitochondrial, Threonyl-tRNA synthetase

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15067-1-PBS targets TARS2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7074

Product name: Recombinant human TARS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 369-718 aa of BC000541

Sequence: EHYQEDMFAVQPPGSDRPPSSQSDDSTRHITDTLALKPMNCPAHCLMFAHRPRSWRELPLRLADFGALHRAEASGGLGGLTRLRCFQQDDAHIFCTTDQLEAEIQSCLDFLRSVYAVLGFSFRLALSTRPSGFLGDPCLWDQAEQVLKQALKEFGEPWDLNSGDGAFYGPKIDVHLHDALGRPHQCGTIQLDFQLPLRFDLQYKGQAGALERPVLIHRAVLGSVERLLGVLAESCGGKWPLWLSPFQVVVIPVGSEQEEYAKEAQQSLRAAGLVSDLDADSGLTLSRRIRRAQLAHYNFQFVVGQKEQSKRTVNIRTRDNRRLGEWDLPEAVQRLVELQNTRVPNAEEIF

相同性解析による交差性が予測される生物種
Full Name threonyl-tRNA synthetase 2, mitochondrial (putative)
Calculated molecular weight 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC000541
Gene Symbol TARS2
Gene ID (NCBI) 80222
RRIDAB_2200668
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BW92
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TARS2 (mitochondrial (mt) threonyl-tRNA synthetase 2) is a class-II aminoacyl tRNA synthetase. Its canonical role is to ligate threonine to its cognate tRNA molecule during mitochondrial protein translation. TARS2 is required for the activation of mTORC1, a master regulator of cell growth and proliferation (PMID: 39509107, 37454282). TARS2 mainly localizes to mitochondria because of its N-terminal mitochondrial targeting sequence (MTS) (PMID: 33340489).

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