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Target

Host

Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

TARS2 Polyclonal antibody

TARS2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15067-1-AP

Synonyms

TARS2, TARSL1, Threonine tRNA ligase, ThrRS

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 15067-1-AP

    WB analysis of HepG2 using 15067-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15067-1-AP (TARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15067-1-AP

    WB analysis of HeLa using 15067-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15067-1-AP (TARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 15067-1-AP

    WB analysis of mouse liver using 15067-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15067-1-AP (TARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 15067-1-AP

    IP experiment of mouse liver using 15067-1-AP

    IP result of anti-TARS2 (IP:15067-1-AP, 4ug; Detection:15067-1-AP 1:600) with mouse liver tissue lysate 4000ug.

  • IHC staining of mouse heart using 15067-1-AP

    IHC staining of mouse heart using 15067-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 15067-1-AP

    IHC staining of mouse heart using 15067-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 15067-1-AP

    IHC staining of mouse liver using 15067-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 15067-1-AP

    IHC staining of mouse liver using 15067-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 15067-1-AP

    IF Staining of HepG2 using 15067-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15067-1-AP (TARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse heart tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15067-1-AP targets TARS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TARS2 fusion protein Ag7074 相同性解析による交差性が予測される生物種
Full Name threonyl-tRNA synthetase 2, mitochondrial (putative)
Calculated molecular weight 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC000541
Gene symbol TARS2
Gene ID (NCBI) 80222
RRIDAB_2200668
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TARS2 antibody 15067-1-APDownload protocol
IHC protocol for TARS2 antibody 15067-1-APDownload protocol
IF protocol for TARS2 antibody 15067-1-APDownload protocol
IP protocol for TARS2 antibody 15067-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF, WB, IP

Mol Cell

Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.

Authors - Sung-Hoon Kim
  • KD Validated
View Article
humanWB

Bioengineered

Knockdown of mitochondrial threonyl-tRNA synthetase 2 inhibits lung adenocarcinoma cell proliferation and induces apoptosis.

Authors - Hui Tian
  • KD Validated
View Article
mouseWB

Redox Biol

Decreased expression of mitochondrial aminoacyl-tRNA synthetases causes downregulation of OXPHOS subunits in type 2 diabetic muscle

Authors - Iliana López-Soldado
View Article
humanIHC

Int J Biol Markers

Potential prognostic biomarkers of hepatocellular carcinoma based on 4D label-free quantitative proteomics analysis pilot investigation

Authors - Lida Suo
View Article