Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HCT 116 cells, THP-1 cells, HepG2 cells, mouse spleen tissue |
Positive IP detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85766-4-RR targets TAP1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | TAP1 fusion protein Ag1619 相同性解析による交差性が予測される生物種 |
Full Name | transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) |
Calculated molecular weight | 81 kDa |
Observed molecular weight | 70-80 kDa |
GenBank accession number | BC014081 |
Gene Symbol | TAP1 |
Gene ID (NCBI) | 6890 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q03518 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TAP1 antibody 85766-4-RR | Download protocol |
IP protocol for TAP1 antibody 85766-4-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |