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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAP1 Recombinant antibody (85766-4-RR)

    WB analysis using 85766-4-RR (same clone as 85766-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85766-4-RR (TAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using TAP1 Recombinant antibody (85766-4-RR)

    WB analysis of THP-1 using 85766-4-RR (same clone as 85766-4-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 85766-4-RR (TAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HCT 116 cells using TAP1 Recombinant antibody (85766-4-RR)

    WB analysis of HCT 116 using 85766-4-RR (same clone as 85766-4-PBS)

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 85766-4-RR (TAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TAP1 Recombinant antibody (85766-4-RR)

    IP experiment of HepG2 using 85766-4-RR (same clone as 85766-4-PBS)

    IP result of anti-TAP1 (IP:85766-4-RR, 4ug; Detection:85766-4-RR 1:1000) with HepG2 cells lysate 1800 ug. This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TAP1 Recombinant antibody (85766-4-RR)

    IHC staining of human pancreas cancer using 85766-4-RR (same clone as 85766-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 85766-4-RR (TAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

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TAP1 Recombinant antibody, PBS Only

TAP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 85766-4-PBS
Clone No.250037E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ABCB2, ATP-binding cassette sub-family B member 2, EC:7.4.2.14, PSF1, PSF-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85766-4-PBS targets TAP1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TAP1 fusion protein Ag1619 相同性解析による交差性が予測される生物種
Full Name transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
Calculated molecular weight 81 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC014081
Gene Symbol TAP1
Gene ID (NCBI) 6890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03518
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.