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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CoraLite® Plus 488-conjugated Syntaxin 6 Polyclona (CL488-10841)

    IF Staining of PC-12 using CL488-10841

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CoraLite® Plus 488 Syntaxin 6 antibody (CL488-10841) at dilution of 1:200, CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of PC-12 cells using CoraLite® Plus 488-conjugated Syntaxin 6 Polyclona (CL488-10841)

    FC experiment of PC-12 using CL488-10841

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Syntaxin 6 (CL488-10841) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Syntaxin 6 Polyclonal antibody

Syntaxin 6 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10841

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

Syntaxin-6, STX6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inPC-12 cells
Positive FC (Intra) detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10841 targets Syntaxin 6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1284

Product name: Recombinant human STX6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-255 aa of BC009944

Sequence: MSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQWCAIAILFAVLLVVLILFLVL

相同性解析による交差性が予測される生物種
Full Name syntaxin 6
Calculated molecular weight 29 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC009944
Gene Symbol Syntaxin 6
Gene ID (NCBI) 10228
RRIDAB_2919003
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43752
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Syntaxin 6 antibody CL488-10841Download protocol
FC protocol for CL Plus 488 Syntaxin 6 antibody CL488-10841Download protocol
Standard Protocols
Click here to view our Standard Protocols