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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Synaptopodin Polyclonal antibody

Synaptopodin Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21064

Synonyms

KIAA1029, synaptopodin, SYNPO

Validation Data Gallery

Applications:
  • IF Staining of rat brain using CL488-21064

    IF Staining of rat brain using CL488-21064

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 Synaptopodin antibody (CL488-21064) at dilution of 1:200.

  • FC experiment of U-87 MG using CL488-21064

    FC experiment of U-87 MG using CL488-21064

    1X10^6 U-87 MG cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Synaptopodin (CL488-21064) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inrat brain tissue
Positive FC detected inU-87 MG cells
For other applications, we recommend the unconjugated version of this antibody, 21064-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-21064 targets Synaptopodin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptopodin fusion protein Ag15089 相同性解析による交差性が予測される生物種
Full Name synaptopodin
Calculated molecular weight 929 aa, 99 kDa
Observed molecular weight 99-115 kDa
GenBank accession numberBC146665
Gene symbol SYNPO
Gene ID (NCBI) 11346
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Synaptopodin antibody CL488-21064Download protocol
Standard Protocols
Click here to view our Standard Protocols