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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Surfactant protein D Polyclonal antibody (11839-1-AP)

    WB analysis using 11839-1-AP (same clone as 11839-1-PBS)

    Mouse lung tissue was subjected to SDS PAGE followed by western blot with 11839-1-AP (Surfactant protein D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11839-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using Surfactant protein D Polyclonal antibody (11839-1-AP)

    IP experiment of mouse lung using 11839-1-AP (same clone as 11839-1-PBS)

    IP result of anti-Surfactant protein D (IP:11839-1-AP, 3ug; Detection:11839-1-AP 1:300) with mouse lung tissue lysate 2800ug. This data was developed using the same antibody clone with 11839-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Surfactant protein D Polyclonal antibody (11839-1-AP)

    IHC staining of mouse lung using 11839-1-AP (same clone as 11839-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11839-1-AP (Surfactant protein D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11839-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Surfactant protein D Polyclonal antibody (11839-1-AP)

    IHC staining of mouse lung using 11839-1-AP (same clone as 11839-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11839-1-AP (Surfactant protein D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11839-1-PBS in a different storage buffer formulation.

Surfactant protein D Polyclonal antibody, PBS Only

Surfactant protein D Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11839-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11839-1-PBS targets Surfactant protein D in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2461

Product name: Recombinant human SFTPD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-375 aa of BC022318

Sequence: AGMKTYSHRTMPSACTLVMCSSVESGLPGRDGRDGREGPRGEKGDPGLPGAAGQAGMPGQAGPVGPKGDNGSVGEPGPKGDTGPSGPPGPPGVPGPAGREGPLGKQGNIGPQGKPGPKGEAGPKGEVGAPGMQGSAGARGLAGPKGERGVPGERGVPGNTGAAGSAGAMGPQGSPGARGPPGLKGDKGIPGDKGAKGESGLPDVASLRQQVEALQGQVQHLQAAFSQYKKVELFPNGQSVGEKIFKTAGFVKPFTEAQLLCTQAGGQLASPRSAAENAALQQLVVAKNEAAFLSMTDSKTEGKFTYPTGESLVYSNWAPGKPNDDGGSEDCVEIFTNGKWNDRACGEKRLVVCEF

相同性解析による交差性が予測される生物種
Full Name surfactant protein D
Calculated molecular weight 375 aa, 38 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC022318
Gene Symbol Surfactant protein D
Gene ID (NCBI) 6441
RRIDAB_2254351
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP35247
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Surfactant protein-D (SP-D) is a pattern recognition molecule of the collectin family of C-type lectins, which consist of four structural domains: a cysteine-rich N-terminal domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain (PMID: 16213021; 15030473). SP-D is primarily expressed and secreted by type II alveolar cells (PMID: 16684127). It plays a central role in the pulmonary host defence and the modulation of allergic responses.

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