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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Surfactant Protein A Polyclonal antibody

Surfactant Protein A Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11850

Synonyms

Alveolar proteinosis protein, COLEC4, Collectin 4, FLJ51913, PSAP, PSP A, PSPA, SFTP1, SFTPA, SFTPA1, SFTPA1B, SP A, SP A1, surfactant protein A1

Validation Data Gallery

Applications:
  • FC experiment of A549 using CL488-11850

    FC experiment of A549 using CL488-11850

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Surfactant Protein A (CL488-11850)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 11850-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-11850 targets Surfactant Protein A in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Surfactant Protein A fusion protein Ag2417 相同性解析による交差性が予測される生物種
Full Name surfactant protein A1
Calculated molecular weight 248 aa, 26 kDa
GenBank accession numberBC026229
Gene symbol SFTPA1
Gene ID (NCBI) 653509
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Surfactant protein A (SP-A), is the major protein component of pulmonary surfactant involved in surfactant-related function or structure and in the regulation of inflammatory processes and innate host defens. In humans and primates, SP-A1 (SFTPA1) and SP-A2 (SFTPA2) genes encode SP-A, and these two SP-A genes have high homology (PMID: 34484180, PMID: 19392648). This antibody can recognize both SP-A1 (SFTPA1) and SP-A2 (SFTPA2).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Surfactant Protein A antibody CL488-11850Download protocol
Standard Protocols
Click here to view our Standard Protocols