Validation Data Gallery
Tested Applications
| Positive WB detected in | 5637 cells, COLO 320 cells, mouse colon tissue, PC-3 cells, MCF-7 cells, rat colon tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87674-7-RR targets Smoothelin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag19983 Product name: Recombinant human SMTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 458-811 aa of BC034237 Sequence: KTTFTIEIKDGRGQASTGRVLLPTGNQRAELTLGLRAPPTLLSTSSGGKSTITRVNSPGTLARLGSVTHVTSFSHAPPSSRGGCSIKMEPEPAEPLAAAVEAANGAEQTRVNKAPEGRSPLSAEELMTIEDEGVLDKMLDQSTDFEERKLIRAALRELRQRKRDQRDKERERRLQEARGRPGEGRGNTATETTTRHSQRAADGSAVSTVTKTERLVHSNDGTRTARTTTVESSFVRRSENGSGSTMMQTKTFSSSSSSKKMGSIFDREDQASPRAGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGAAGSPGGPRAAVQRSTSFGVPNANSIKQMLLDWCRA 相同性解析による交差性が予測される生物種 |
| Full Name | smoothelin |
| Calculated molecular weight | 940 aa, 102 kDa |
| Observed molecular weight | 59 kDa and 117 kDa |
| GenBank accession number | BC034237 |
| Gene Symbol | SMTN |
| Gene ID (NCBI) | 6525 |
| RRID | AB_3745689 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P53814 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Smoothelin is a cytoskeletal-associated protein expressed specifically in differentiated smooth muscle cells (SMCs). It exists in two main isoforms: the ~59 kDa Smoothelin-A in visceral SMCs and the ~110 kDa Smoothelin-B in vascular SMCs, generated through a dual-promoter system and alternative splicing. Smoothelin serves as a reliable marker of contractile SMCs and helps maintain the contractile phenotype by interacting with actin filaments and other cytoskeletal components. This antibody reacts with the 59 kDa and 117 kDa bands, corresponding to isoforms A and B, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Smoothelin antibody 87674-7-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |