• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Smoothelin Recombinant monoclonal antibody (87674-7-RR)

    WB analysis using 87674-7-RR (same clone as 87674-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87674-7-RR (Smoothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87674-7-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Smoothelin Recombinant monoclonal antibody (87674-7-RR)

    WB analysis using 87674-7-RR (same clone as 87674-7-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 87674-7-RR (Smoothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87674-7-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Smoothelin Recombinant monoclonal antibody (87674-7-RR)

    WB analysis using 87674-7-RR (same clone as 87674-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87674-7-RR (Smoothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87674-7-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using Smoothelin Recombinant monoclonal antibody (87674-7-RR)

    WB analysis of COLO 320 using 87674-7-RR (same clone as 87674-7-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 87674-7-RR (Smoothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87674-7-PBS in a different storage buffer formulation.

  • Affinity of 87674-7-RR

    Affinity of 87674-7-RR

    Biolayer interferometry (BLI) kinetic assays of 87674-7-RR against Human Smoothelin were performed. The affinity constant is 0.257 nM.

Smoothelin Recombinant monoclonal antibody, PBS Only

Smoothelin Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87674-7-PBS
Clone No.253233C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

SMTN, SMSMO

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87674-7-PBS targets Smoothelin in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19983

Product name: Recombinant human SMTN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 458-811 aa of BC034237

Sequence: KTTFTIEIKDGRGQASTGRVLLPTGNQRAELTLGLRAPPTLLSTSSGGKSTITRVNSPGTLARLGSVTHVTSFSHAPPSSRGGCSIKMEPEPAEPLAAAVEAANGAEQTRVNKAPEGRSPLSAEELMTIEDEGVLDKMLDQSTDFEERKLIRAALRELRQRKRDQRDKERERRLQEARGRPGEGRGNTATETTTRHSQRAADGSAVSTVTKTERLVHSNDGTRTARTTTVESSFVRRSENGSGSTMMQTKTFSSSSSSKKMGSIFDREDQASPRAGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGAAGSPGGPRAAVQRSTSFGVPNANSIKQMLLDWCRA

相同性解析による交差性が予測される生物種
Full Name smoothelin
Calculated molecular weight 940 aa, 102 kDa
Observed molecular weight59 kDa and 117 kDa
GenBank accession numberBC034237
Gene Symbol SMTN
Gene ID (NCBI) 6525
RRIDAB_3745689
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP53814
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Smoothelin is a cytoskeletal-associated protein expressed specifically in differentiated smooth muscle cells (SMCs). It exists in two main isoforms: the ~59 kDa Smoothelin-A in visceral SMCs and the ~110 kDa Smoothelin-B in vascular SMCs, generated through a dual-promoter system and alternative splicing. Smoothelin serves as a reliable marker of contractile SMCs and helps maintain the contractile phenotype by interacting with actin filaments and other cytoskeletal components. This antibody reacts with the 59 kDa and 117 kDa bands, corresponding to isoforms A and B, respectively.

||
New chat

Able™

正在加载，请稍候...