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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Septin 8 Monoclonal antibody (66188-1-Ig)

    WB analysis of HeLa using 66188-1-Ig (same clone as 66188-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66188-1-Ig (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Septin 8 Monoclonal antibody (66188-1-Ig)

    IHC staining of mouse brain using 66188-1-Ig (same clone as 66188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Septin 8 Monoclonal antibody (66188-1-Ig)

    IHC staining of mouse brain using 66188-1-Ig (same clone as 66188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Septin 8 Monoclonal antibody (66188-1-Ig)

    IHC staining of human gliomas using 66188-1-Ig (same clone as 66188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Septin 8 Monoclonal antibody (66188-1-Ig)

    IHC staining of human gliomas using 66188-1-Ig (same clone as 66188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Septin 8 Monoclonal antibody (66188-1-Ig)

    IF Staining of HeLa using 66188-1-Ig (same clone as 66188-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 8 antibody (66188-1-Ig, Clone: 4A12G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Septin 8 Monoclonal antibody (66188-1-Ig)

    IF Staining of HeLa using 66188-1-Ig (same clone as 66188-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 8 antibody (66188-1-Ig, Clone: 4A12G9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

Septin 8 Monoclonal antibody, PBS Only

Septin 8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66188-1-PBS
Clone No.4A12G9

Host / Isotype

Mouse / IgM

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SEPT8, KIAA0202, SEP2, SEPTIN8, Septin-8

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66188-1-PBS targets Septin 8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19114

Product name: Recombinant human SEPT8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-256 aa of BC001329

Sequence: MKKLDSKVNIIPIIAKADTISKSELHKFKIKIMGELVSNGVQIYQFPTDDEAVAEINAVMNAHLPFAVVGSTEEVKVGNKLVRARQYPWGVVQVENENHCDFVKLREMLIRVNMEDLREQTHSRHYELYRRCKLEEMGFQDSDGDSQPFSLQETYEAKRKEFLSELQRKEEEMRQMFVNKVKETELELKEKERELHEKFEHLKRVHQEEKRKVEEKRRELEEETNAFNRRKAAVEALQSQALHATSQQPLRKDKDK

相同性解析による交差性が予測される生物種
Full Name septin 8
Calculated molecular weight55.6 kDa
Observed molecular weight 50-58 kDa
GenBank accession numberBC001329
Gene Symbol Septin 8
Gene ID (NCBI) 23176
RRIDAB_2881583
Conjugate Unconjugated
Form
FormLiquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDQ92599
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are membrane-associated GTPases which are involved in cytokinesis and cellular morphogenesis. Septin 8 is an interaction partner of Septin 4. It may play a role in platelet secretion.

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