Septin 8 Monoclonal antibody
Septin 8 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgM
Reactivity
human, mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
4A12G9
Cat no : 66188-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells |
Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
66188-1-Ig targets Septin 8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgM |
Class | Monoclonal |
Type | Antibody |
Immunogen | Septin 8 fusion protein Ag19114 相同性解析による交差性が予測される生物種 |
Full Name | septin 8 |
Calculated molecular weight | 55.6 kDa |
Observed molecular weight | 50-58 kDa |
GenBank accession number | BC001329 |
Gene symbol | SEPT8 |
Gene ID (NCBI) | 23176 |
RRID | AB_2881583 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Thiophilic affinity chromatograph |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are membrane-associated GTPases which are involved in cytokinesis and cellular morphogenesis. Septin 8 is an interaction partner of Septin 4. It may play a role in platelet secretion.
Protocols
Product Specific Protocols | |
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WB protocol for Septin 8 antibody 66188-1-Ig | Download protocol |
IHC protocol for Septin 8 antibody 66188-1-Ig | Download protocol |
IF protocol for Septin 8 antibody 66188-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |