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Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood leukocyte using P-Selectin/CD62P Recombinant antibody (83947-5-RR)

    WB analysis of human peripheral blood leukocyte using 83947-5-RR (same clone as 83947-5-PBS)

    human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using P-Selectin/CD62P Recombinant antibody (83947-5-RR)

    IHC staining of rat spleen using 83947-5-RR (same clone as 83947-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using P-Selectin/CD62P Recombinant antibody (83947-5-RR)

    IHC staining of rat spleen using 83947-5-RR (same clone as 83947-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using P-Selectin/CD62P Recombinant antibody (83947-5-RR)

    IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using P-Selectin/CD62P Recombinant antibody (83947-5-RR)

    IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using P-Selectin/CD62P Recombinant antibody (83947-5-RR)

    IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using P-Selectin/CD62P Recombinant antibody (83947-5-RR)

    IF Staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using P-Selectin/CD62P antibody (83947-5-RR, Clone: 240766D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using P-Selectin/CD62P Recombinant antibody (83947-5-RR)

    IF Staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using P-Selectin/CD62P antibody (83947-5-RR, Clone: 240766D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83947-5-RR

    Affinity and Kinetic Characterization of 83947-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83947-5-RR against Mouse P-Selectin/CD62P were performed. The affinity constant is 7.97 nM.

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Mouse P-Selectin/CD62P Recombinant antibody, PBS Only

P-Selectin/CD62P Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83947-5-PBS
Clone No.240766D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

P-selectin, CD62P, 240766D3, PADGEM, P selectin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83947-5-PBS targets P-Selectin/CD62P in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name selectin, platelet
Calculated molecular weight83 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_011347.2
Gene Symbol Selp
Gene ID (NCBI) 20344
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ01102
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.