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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Secretogranin III Polyclonal antibody

Secretogranin III Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10954

Synonyms

FLJ90833, SCG3, Secretogranin 3, secretogranin III, SGIII

Validation Data Gallery

Applications:
  • IF Staining of mouse pancreas using CL488-10954

    IF Staining of mouse pancreas using CL488-10954

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CoraLite® Plus 488 Secretogranin III antibody (CL488-10954) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using CL488-10954

    IF Staining of mouse pancreas using CL488-10954

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CoraLite® Plus 488 Secretogranin III antibody (CL488-10954) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IF detected inmouse pancreas tissue
For other applications, we recommend the unconjugated version of this antibody, 10954-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-10954 targets Secretogranin III in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Secretogranin III fusion protein Ag1397 相同性解析による交差性が予測される生物種
Full Name secretogranin III
Calculated molecular weight 53 kDa
GenBank accession numberBC014539
Gene symbol SCG3
Gene ID (NCBI) 29106
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Secretogranin III (SCG3), a member of the granin protein family, is expressed specifically in neuronal and endocrine cells. SCG3 was synthesized as a 61- or 63-kDa protein and processed to a 48-kDa form which, in turn, was partially cleaved to fragments of 28 and 20 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Secretogranin III antibody CL488-10954Download protocol
Standard Protocols
Click here to view our Standard Protocols